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Literature summary for 2.4.2.54 extracted from

  • Dumitru, R.V.; Ragsdale, S.W.
    Mechanism of 4-(beta-D-ribofuranosyl)aminobenzene 5'-phosphate synthase, a key enzyme in the methanopterin biosynthetic pathway (2004), J. Biol. Chem., 279, 39389-39395.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Escherichia coli Methanocaldococcus jannaschii

Inhibitors

Inhibitors Comment Organism Structure
4-(beta-D-ribofuranosyl)aminobenzene 5'-phosphate uncompetitive inhibition under varied 5-phospho-alpha-D-ribose 1-diphosphate and saturated 4-aminobenzoate or under varied 4-aminobenzoate and saturated 5-phospho-alpha-D-ribose 1-diphosphate Methanocaldococcus jannaschii
CO2 uncompetitive inhibition under varied 5-phospho-alpha-D-ribose 1-diphosphate and saturated 4-aminobenzoate. Mixed type of inhibition under varied 4-aminobenzoate and saturated 5-phospho-alpha-D-ribose 1-diphosphate Methanocaldococcus jannaschii
diphosphate competitive inhibition under saturated 5-phospho-alpha-D-ribose 1-diphosphate and varied 4-aminobenzoate. Mixed type of inhibition under varied 4-aminobenzoate and saturated 5-phospho-alpha-D-ribose 1-diphosphate Methanocaldococcus jannaschii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.15
-
4-Aminobenzoate pH 4.8, 70°C Methanocaldococcus jannaschii
1.5
-
5-phospho-alpha-D-ribose 1-diphosphate pH 4.8, 70°C Methanocaldococcus jannaschii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36200
-
2 * 36200, SDS-PAGE Methanocaldococcus jannaschii
70000
-
gel filtration Methanocaldococcus jannaschii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5-phospho-alpha-D-ribose 1-diphosphate + 4-aminobenzoate Methanocaldococcus jannaschii the enzyme is involved in biosynthesis of tetrahydromethanopterin, a folate analog involved in the C1 metabolism of methanogenic archaea, sulfate-reducing archaea, and methylotrophic bacteria 4-(beta-D-ribofuranosyl)aminobenzene 5'-phosphate + CO2 + diphosphate
-
?

Organism

Organism UniProt Comment Textmining
Methanocaldococcus jannaschii Q58822
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Methanocaldococcus jannaschii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.248
-
pH 4.8, 70°C Methanocaldococcus jannaschii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-phospho-alpha-D-ribose 1-diphosphate + 4-aminobenzoate the enzyme is involved in biosynthesis of tetrahydromethanopterin, a folate analog involved in the C1 metabolism of methanogenic archaea, sulfate-reducing archaea, and methylotrophic bacteria Methanocaldococcus jannaschii 4-(beta-D-ribofuranosyl)aminobenzene 5'-phosphate + CO2 + diphosphate
-
?
5-phospho-alpha-D-ribose 1-diphosphate + 4-aminobenzoate ordered Bi-Ter mechanism involving binding of 5-phospho-alpha-D-ribose 1-diphosphate, then 4-aminobenzoate, followed by release of the products CO2, then 4-(beta-D-ribofuranosyl)aminobenzene-5'-phosphate, and finally diphosphate Methanocaldococcus jannaschii 4-(beta-D-ribofuranosyl)aminobenzene 5'-phosphate + CO2 + diphosphate
-
?

Subunits

Subunits Comment Organism
homodimer 2 * 36200, SDS-PAGE Methanocaldococcus jannaschii

Synonyms

Synonyms Comment Organism
beta-RFA-P synthase
-
Methanocaldococcus jannaschii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
assay at Methanocaldococcus jannaschii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.23
-
5-phospho-alpha-D-ribose 1-diphosphate pH 4.8, 70°C Methanocaldococcus jannaschii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.9
-
-
Methanocaldococcus jannaschii

Cofactor

Cofactor Comment Organism Structure
additional information the enzyme lacks any chromogenic cofactor, and the presence of pyridoxal phosphate and the mechanistically related pyruvoyl cofactors is excluded Methanocaldococcus jannaschii

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.21
-
4-(beta-D-ribofuranosyl)aminobenzene 5'-phosphate pH 4.8, 70°C, uncompetitive inhibition under varied 5-phospho-alpha-D-ribose 1-diphosphate and saturated 4-aminobenzoate Methanocaldococcus jannaschii
0.3
-
4-(beta-D-ribofuranosyl)aminobenzene 5'-phosphate pH 4.8, 70°C, uncompetitive inhibition under varied 4-aminobenzoate and saturated 5-phospho-alpha-D-ribose 1-diphosphate Methanocaldococcus jannaschii
0.32
-
diphosphate pH 4.8, 70°C, competitive inhibition under saturated 5-phospho-alpha-D-ribose 1-diphosphate and varied 4-aminobenzoate Methanocaldococcus jannaschii
0.99
-
CO2 pH 4.8, 70°C, uncompetitive inhibition under varied 5-phospho-alpha-D-ribose 1-diphosphate and saturated 4-aminobenzoate Methanocaldococcus jannaschii