Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli | Methanocaldococcus jannaschii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-(beta-D-ribofuranosyl)aminobenzene 5'-phosphate | uncompetitive inhibition under varied 5-phospho-alpha-D-ribose 1-diphosphate and saturated 4-aminobenzoate or under varied 4-aminobenzoate and saturated 5-phospho-alpha-D-ribose 1-diphosphate | Methanocaldococcus jannaschii | |
CO2 | uncompetitive inhibition under varied 5-phospho-alpha-D-ribose 1-diphosphate and saturated 4-aminobenzoate. Mixed type of inhibition under varied 4-aminobenzoate and saturated 5-phospho-alpha-D-ribose 1-diphosphate | Methanocaldococcus jannaschii | |
diphosphate | competitive inhibition under saturated 5-phospho-alpha-D-ribose 1-diphosphate and varied 4-aminobenzoate. Mixed type of inhibition under varied 4-aminobenzoate and saturated 5-phospho-alpha-D-ribose 1-diphosphate | Methanocaldococcus jannaschii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.15 | - |
4-Aminobenzoate | pH 4.8, 70°C | Methanocaldococcus jannaschii | |
1.5 | - |
5-phospho-alpha-D-ribose 1-diphosphate | pH 4.8, 70°C | Methanocaldococcus jannaschii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36200 | - |
2 * 36200, SDS-PAGE | Methanocaldococcus jannaschii |
70000 | - |
gel filtration | Methanocaldococcus jannaschii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-phospho-alpha-D-ribose 1-diphosphate + 4-aminobenzoate | Methanocaldococcus jannaschii | the enzyme is involved in biosynthesis of tetrahydromethanopterin, a folate analog involved in the C1 metabolism of methanogenic archaea, sulfate-reducing archaea, and methylotrophic bacteria | 4-(beta-D-ribofuranosyl)aminobenzene 5'-phosphate + CO2 + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanocaldococcus jannaschii | Q58822 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Methanocaldococcus jannaschii |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.248 | - |
pH 4.8, 70°C | Methanocaldococcus jannaschii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-phospho-alpha-D-ribose 1-diphosphate + 4-aminobenzoate | the enzyme is involved in biosynthesis of tetrahydromethanopterin, a folate analog involved in the C1 metabolism of methanogenic archaea, sulfate-reducing archaea, and methylotrophic bacteria | Methanocaldococcus jannaschii | 4-(beta-D-ribofuranosyl)aminobenzene 5'-phosphate + CO2 + diphosphate | - |
? | |
5-phospho-alpha-D-ribose 1-diphosphate + 4-aminobenzoate | ordered Bi-Ter mechanism involving binding of 5-phospho-alpha-D-ribose 1-diphosphate, then 4-aminobenzoate, followed by release of the products CO2, then 4-(beta-D-ribofuranosyl)aminobenzene-5'-phosphate, and finally diphosphate | Methanocaldococcus jannaschii | 4-(beta-D-ribofuranosyl)aminobenzene 5'-phosphate + CO2 + diphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 36200, SDS-PAGE | Methanocaldococcus jannaschii |
Synonyms | Comment | Organism |
---|---|---|
beta-RFA-P synthase | - |
Methanocaldococcus jannaschii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
assay at | Methanocaldococcus jannaschii |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.23 | - |
5-phospho-alpha-D-ribose 1-diphosphate | pH 4.8, 70°C | Methanocaldococcus jannaschii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.9 | - |
- |
Methanocaldococcus jannaschii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme lacks any chromogenic cofactor, and the presence of pyridoxal phosphate and the mechanistically related pyruvoyl cofactors is excluded | Methanocaldococcus jannaschii |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.21 | - |
4-(beta-D-ribofuranosyl)aminobenzene 5'-phosphate | pH 4.8, 70°C, uncompetitive inhibition under varied 5-phospho-alpha-D-ribose 1-diphosphate and saturated 4-aminobenzoate | Methanocaldococcus jannaschii | |
0.3 | - |
4-(beta-D-ribofuranosyl)aminobenzene 5'-phosphate | pH 4.8, 70°C, uncompetitive inhibition under varied 4-aminobenzoate and saturated 5-phospho-alpha-D-ribose 1-diphosphate | Methanocaldococcus jannaschii | |
0.32 | - |
diphosphate | pH 4.8, 70°C, competitive inhibition under saturated 5-phospho-alpha-D-ribose 1-diphosphate and varied 4-aminobenzoate | Methanocaldococcus jannaschii | |
0.99 | - |
CO2 | pH 4.8, 70°C, uncompetitive inhibition under varied 5-phospho-alpha-D-ribose 1-diphosphate and saturated 4-aminobenzoate | Methanocaldococcus jannaschii |