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Literature summary for 2.4.2.5 extracted from

  • Acosta, J.; Del Arco, J.; Pisabarro, V.; Gago, F.; Fernandez-Lucas, J.
    N-Ribosyltransferase from Archaeoglobus veneficus a novel halotolerant and thermostable biocatalyst for the synthesis of purine ribonucleoside analogs (2020), Front. Bioeng. Biotechnol., 8, 593 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.4.2.5

Application

Application Comment Organism
synthesis the enzyme is a thermophilic and halotolerant biocatalyst that is successfully employed in the synthesis of different purine ribonucleoside analogues, potential of AvNRT as an industrial biocatalyst for the synthesis of nucleoside analogues Archaeoglobus veneficus

Cloned(Commentary)

Cloned (Comment) Organism
gene nrt, sequence comparisons, recombinant expression of N-terminally His6-tagged enzyme AvNRT in Escherichia coli strain BL21(DE3) Archaeoglobus veneficus

Inhibitors

Inhibitors Comment Organism Structure
acetone 50% inhibition at 20% v/v Archaeoglobus veneficus
acetonitril 30% inhibition at 20% v/v Archaeoglobus veneficus
chloroform 20% inhibition at 20% v/v Archaeoglobus veneficus
dimethylformamide complete inhibition at 20% v/v Archaeoglobus veneficus
DMSO 80% inhibition at 20% v/v Archaeoglobus veneficus
ethanol 35% inhibition at 20% v/v Archaeoglobus veneficus
ethyl acetate 20% inhibition at 20% v/v Archaeoglobus veneficus
ethylene glycol 80% inhibition at 20% v/v Archaeoglobus veneficus
glycerol 35% inhibition at 20% v/v Archaeoglobus veneficus
Isopropanol 20% inhibition at 20% v/v Archaeoglobus veneficus
methanol 45% inhibition at 20% v/v Archaeoglobus veneficus
Propylene glycol 78% inhibition at 20% v/v Archaeoglobus veneficus

Metals/Ions

Metals/Ions Comment Organism Structure
NaCl the activity of AvNRT increases up to 2fold in 4 M NaCl aqueous solution Archaeoglobus veneficus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-ribosyl-base1 + base2 Archaeoglobus veneficus
-
 D-ribosyl-base2 + base1
-
 ?
D-ribosyl-base1 + base2 Archaeoglobus veneficus DSM 11195
-
 D-ribosyl-base2 + base1
-
 ?
D-ribosyl-base1 + base2 Archaeoglobus veneficus SNP6
-
 D-ribosyl-base2 + base1
-
 ?

Organic Solvent Stability

Organic Solvent Comment Organism
additional information the enzyme activity is retained in the presence of several water-miscible organic solvents Archaeoglobus veneficus

Organism

Organism UniProt Comment Textmining
Archaeoglobus veneficus F2KPY9 a chemolithoautotrophic hyperthermophilic sulfitereducing archaeal coccus
-
Archaeoglobus veneficus DSM 11195 F2KPY9 a chemolithoautotrophic hyperthermophilic sulfitereducing archaeal coccus
-
Archaeoglobus veneficus SNP6 F2KPY9 a chemolithoautotrophic hyperthermophilic sulfitereducing archaeal coccus
-

Purification (Commentary)

Purification (Comment) Organism
recombinant N-terminally His6-tagged enzyme AvNRT from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and preparative gel filtration Archaeoglobus veneficus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-ribosyl-base1 + base2
-
 Archaeoglobus veneficus D-ribosyl-base2 + base1
-
 ?
D-ribosyl-base1 + base2
-
 Archaeoglobus veneficus DSM 11195 D-ribosyl-base2 + base1
-
 ?
D-ribosyl-base1 + base2
-
 Archaeoglobus veneficus SNP6 D-ribosyl-base2 + base1
-
 ?
guanosine + 2,6-diaminopurine
-
 Archaeoglobus veneficus guanine + 2,6-diaminopurine ribonucleoside
-
 ?
guanosine + 2,6-diaminopurine
-
 Archaeoglobus veneficus DSM 11195 guanine + 2,6-diaminopurine ribonucleoside
-
 ?
guanosine + 2,6-diaminopurine
-
 Archaeoglobus veneficus SNP6 guanine + 2,6-diaminopurine ribonucleoside
-
 ?
guanosine + 2-chloroadenine
-
 Archaeoglobus veneficus guanine + 2-chloroadenosine
-
 ?
guanosine + 2-chloroadenine
-
 Archaeoglobus veneficus DSM 11195 guanine + 2-chloroadenosine
-
 ?
guanosine + 2-chloroadenine
-
 Archaeoglobus veneficus SNP6 guanine + 2-chloroadenosine
-
 ?
guanosine + 2-fluoroadenine
-
 Archaeoglobus veneficus guanine + 2-fluoroadenosine
-
 ?
guanosine + 2-fluoroadenine
-
 Archaeoglobus veneficus DSM 11195 guanine + 2-fluoroadenosine
-
 ?
guanosine + 2-fluoroadenine
-
 Archaeoglobus veneficus SNP6 guanine + 2-fluoroadenosine
-
 ?
guanosine + 6-methoxyguanine
-
 Archaeoglobus veneficus guanine + 6-methoxyguanosine
-
 ?
guanosine + adenine
-
 Archaeoglobus veneficus guanine + adenosine
-
 ?
guanosine + hypoxanthine
-
 Archaeoglobus veneficus guanine + inosine
-
 ?
additional information substrate specificity of recombinant enzyme, overview. Binding structure of guanosine to the active site of AvNRT Archaeoglobus veneficus ?
-
-
additional information substrate specificity of recombinant enzyme, overview. Binding structure of guanosine to the active site of AvNRT Archaeoglobus veneficus DSM 11195 ?
-
-
additional information substrate specificity of recombinant enzyme, overview. Binding structure of guanosine to the active site of AvNRT Archaeoglobus veneficus SNP6 ?
-
-

Subunits

Subunits Comment Organism
homotetramer 4 * 18000, recombinant enzyme, SDS-PAGE, 4* 19245, recombinant enzyme, analytical ultracentrifugation Archaeoglobus veneficus
More three-dimensional modeling of AvNRT, overview Archaeoglobus veneficus

Synonyms

Synonyms Comment Organism
AvNRT
-
 Archaeoglobus veneficus
N-ribosyltransferase
-
 Archaeoglobus veneficus
NRT
-
 Archaeoglobus veneficus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
80
-
-
 Archaeoglobus veneficus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 100 activity range, profile overview Archaeoglobus veneficus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60 80 the enzyme shows high stability at high temperatures Archaeoglobus veneficus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Archaeoglobus veneficus

pH Range

pH Minimum pH Maximum Comment Organism
4 8 activity range, profile overview Archaeoglobus veneficus

General Information

General Information Comment Organism
additional information enzyme homology structure modeling and molecular dynamics simulations, overview. Nucleophilic attack on C1' by the carboxylate of the catalytic residue Glu85. Pro11 and Pro42 play a prominent role in making up the active site cavity where one of the Asp79 carboxylate oxygens and the carboxamide nitrogen of Asn113 recognize the O5' of the sugar moiety of the substrate while the backbone NH of Ala10 hydrogen bonds to O3'. The heteroaromatic ring of the nucleobase thus gets sandwiched between the phenyl rings of Phe12 and Phe13, on one side and the hydrophobic sidechains of Phe56 and Leu119 on the opposite side. The Met120 side chain sulfur appears close to the H1' atom, which suggests that it likely plays a role, together with the carboxylate of Asp62, in stabilization of the oxocarbenium reaction intermediate Archaeoglobus veneficus