Literature summary for 2.4.2.48 extracted from

Kawamura, T.; Hirata, A.; Ohno, S.; Nomura, Y.; Nagano, T.; Nameki, N.; Yokogawa, T.; Hori, H.
Multisite-specific archaeosine tRNA-guanine transglycosylase (ArcTGT) from Thermoplasma acidophilum, a thermo-acidophilic archaeon (2015), Nucleic Acids Res., 44, 1894-1908 .

Cloned(Commentary)

Cloned (Comment)	Organism
because the Thermoplasma acidophilum enzyme can not be expressed as a soluble protein in Escherichia coli, an expression system using another thermophilic archaeon, Thermococcus kodakarensis is employed. The arcTGT gene in Thermococcus kodakarensis is disrupted, complemented with the Thermoplasma acidophilum arcTGT gene, and tRNALeu variants are expressed. Mass spectrometry analysis of purified tRNALeu variants reveals the modifications of archaeosine13 and archaeosine15 in the wild-type tRNALeu	Thermoplasma acidophilum
expressed in E. coli BL21 (DE3) Rosetta 2 strain	Pyrococcus horikoshii

Cloned (Comment)

Organism

because the Thermoplasma acidophilum enzyme can not be expressed as a soluble protein in Escherichia coli, an expression system using another thermophilic archaeon, Thermococcus kodakarensis is employed. The arcTGT gene in Thermococcus kodakarensis is disrupted, complemented with the Thermoplasma acidophilum arcTGT gene, and tRNALeu variants are expressed. Mass spectrometry analysis of purified tRNALeu variants reveals the modifications of archaeosine13 and archaeosine15 in the wild-type tRNALeu

Thermoplasma acidophilum

expressed in E. coli BL21 (DE3) Rosetta 2 strain

Pyrococcus horikoshii

Organism

Organism	UniProt	Comment	Textmining
Pyrococcus horikoshii	O58843	-	-
Pyrococcus horikoshii OT-3	O58843	-	-
Thermoplasma acidophilum	Q9HI54	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Thermoplasma acidophilum

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
guanine13 in tRNA(Leu) + 7-cyano-7-carbaguanine	-	Thermoplasma acidophilum	7-cyano-7-carbaguanine13 in tRNA(Leu) + guanine	-	?
guanine15 in tRNA(Leu) + 7-cyano-7-carbaguanine	-	Pyrococcus horikoshii	7-cyano-7-carbaguanine15 in tRNA(Leu) + guanine	-	?
guanine15 in tRNA(Leu) + 7-cyano-7-carbaguanine	-	Thermoplasma acidophilum	7-cyano-7-carbaguanine15 in tRNA(Leu) + guanine	-	?
guanine15 in tRNA(Leu) + 7-cyano-7-carbaguanine	-	Pyrococcus horikoshii OT-3	7-cyano-7-carbaguanine15 in tRNA(Leu) + guanine	-	?

Synonyms

Synonyms	Comment	Organism
ArcTGT	-	Pyrococcus horikoshii
ArcTGT	-	Thermoplasma acidophilum
multisite-specific archaeosine tRNA-guanine transglycosylase	-	Thermoplasma acidophilum
Ta1493	-	Thermoplasma acidophilum