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Literature summary for 2.4.2.48 extracted from

  • Nomura, Y.; Onda, Y.; Ohno, S.; Taniguchi, H.; Ando, K.; Oka, N.; Nishikawa, K.; Yokogawa, T.
    Purification and comparison of native and recombinant tRNA-guanine transglycosylases from Methanosarcina acetivorans (2013), Protein Expr. Purif., 88, 13-19.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli ER2566 cells Methanosarcina acetivorans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0012
-
guanine15 in tRNA native enzyme, at pH 7.5 and 37°C Methanosarcina acetivorans
0.0021
-
guanine15 in tRNA recombinant enzyme, at pH 7.5 and 37°C Methanosarcina acetivorans

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ maximum activity at 10 mM Mg2+ Methanosarcina acetivorans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
19000
-
-
Methanosarcina acetivorans
54000
-
-
Methanosarcina acetivorans
150000
-
gel filtration Methanosarcina acetivorans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
guanine15 in tRNA + 7-cyano-7-carbaguanine Methanosarcina acetivorans
-
7-cyano-7-carbaguanine15 in tRNA + guanine
-
?
guanine15 in tRNA + 7-cyano-7-carbaguanine Methanosarcina acetivorans DSM 2834
-
7-cyano-7-carbaguanine15 in tRNA + guanine
-
?

Organism

Organism UniProt Comment Textmining
Methanosarcina acetivorans Q8THU2
-
-
Methanosarcina acetivorans DSM 2834 Q8THU2
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, phenyl Sepharose column chromatography, HiTrap Q column chromatography, HiTrap heparin column chromatography, Mono Q column chromatography, and Sephacryl S-200 gel filtration Methanosarcina acetivorans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
27
-
native enzyme, after 2900fold purification, at pH 7.5 and 37°C Methanosarcina acetivorans
31
-
recombinant enzyme, after 14fold purification, at pH 7.5 and 37°C Methanosarcina acetivorans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
guanine15 in tRNA + 7-cyano-7-carbaguanine
-
Methanosarcina acetivorans 7-cyano-7-carbaguanine15 in tRNA + guanine
-
?
guanine15 in tRNA + 7-cyano-7-carbaguanine
-
Methanosarcina acetivorans DSM 2834 7-cyano-7-carbaguanine15 in tRNA + guanine
-
?

Subunits

Subunits Comment Organism
heterotetramer 2 * 54000 + 2 * 19000 Methanosarcina acetivorans

Synonyms

Synonyms Comment Organism
archaeosine tRNAguanine transglycosylase
-
Methanosarcina acetivorans
ArcTGT
-
Methanosarcina acetivorans

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0311
-
guanine15 in tRNA native enzyme, at pH 7.5 and 37°C Methanosarcina acetivorans
0.0667
-
guanine15 in tRNA recombinant enzyme, at pH 7.5 and 37°C Methanosarcina acetivorans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
Methanosarcina acetivorans

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
27
-
guanine15 in tRNA native enzyme, at pH 7.5 and 37°C Methanosarcina acetivorans
31
-
guanine15 in tRNA recombinant enzyme, at pH 7.5 and 37°C Methanosarcina acetivorans