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Literature summary for 2.4.2.38 extracted from

  • Bondili, J.S.; Castilho, A.; Mach, L.; Gloessl, J.; Steinkellner, H.; Altmann, F.; Strasser, R.
    Molecular cloning and heterologous expression of beta1,2-xylosyltransferase and core alpha1,3-fucosyltransferase from maize (2006), Phytochemistry, 67, 2215-2224.
    View publication on PubMed

Application

Application Comment Organism
synthesis potential of maize and the beta1,2-xylosyltransferase as a production system for heterologous glycoproteins Zea mays

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, genetic organization and structure, functional expression in Spodoptera frugiperda Sf21 cells using the baculovirus transfection system Zea mays

Protein Variants

Protein Variants Comment Organism
additional information targeted downregulation of the enzyme and the alpha1,3-fucosyltransferase allow production of heterologous proteins in a maize expression system Zea mays

Inhibitors

Inhibitors Comment Organism Structure
Mn2+ above 10 mM Zea mays

Metals/Ions

Metals/Ions Comment Organism Structure
additional information is active in the presence of 10 mM EDTA and does not require the addition of any metal ions for activity Zea mays

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
56000
-
x * 56000, recombinant enzyme, SDS-PAGE Zea mays

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Zea mays the enzyme is involved in the modification of asparagine-linked oligosaccharides of maize glycoproteins with beta1,2-xylose sugar residues ?
-
?

Organism

Organism UniProt Comment Textmining
Zea mays Q2UVB3 gene xt1; cv. Monalisa
-

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Zea mays
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme is involved in the modification of asparagine-linked oligosaccharides of maize glycoproteins with beta1,2-xylose sugar residues Zea mays ?
-
?
additional information the recombinant enzyme is able to convert a mammalian acceptor glycoprotein into an antigen binding anti-plant N-glycan antibodies, overview Zea mays ?
-
?
pyridylaminated-GnGn glycopeptide + UDP-xylose
-
Zea mays ?
-
?

Subunits

Subunits Comment Organism
? x * 56000, recombinant enzyme, SDS-PAGE Zea mays

Synonyms

Synonyms Comment Organism
beta1,2-xylosyltransferase
-
Zea mays
XylT
-
Zea mays

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Zea mays

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
assay at Zea mays