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Literature summary for 2.4.2.38 extracted from

  • Bencur, P.; Steinkellner, H.; Svoboda, B.; Mucha, J.; Strasser, R.; Kolarich, D.; Hann, S.; Koellensperger, G.; Gloessl, J.; Altmann, F.; Mach, L.
    Arabidopsis thaliana beta1,2-xylosyltransferase: an unusual glycosyltransferase with the potential to act at multiple stages of the plant N-glycosylation pathway (2005), Biochem. J., 388, 515-525.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type and mutant enzymes in Spodoptera frugiperda SF21 cells using the baculovirus infection system Arabidopsis thaliana

Protein Variants

Protein Variants Comment Organism
additional information construction of several N-terminal deletion mutants, the first 54 residues can be deleted without affecting the catalytic activity of the enzyme, removal of an additional five amino acids lead to the formation of an inactive protein Arabidopsis thaliana

Inhibitors

Inhibitors Comment Organism Structure
Co2+ 59% inhibition at 10 mM Arabidopsis thaliana
EDTA 20% inhibition at 10 mM Arabidopsis thaliana
Ni2+ 99% inhibition at 10 mM Arabidopsis thaliana
UDP competitive Arabidopsis thaliana
UDP-glucose
-
Arabidopsis thaliana
Zn2+ 95% inhibition at 10 mM Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.13
-
UDP-xylose pH 7.0, 37°C, recombinant mutant DELTA39 XylT Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
Golgi apparatus
-
Arabidopsis thaliana 5794
-

Metals/Ions

Metals/Ions Comment Organism Structure
additional information no requirements for metal ions Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Arabidopsis thaliana the enzyme is involved in the biosynthesis of glycoprotein-bound N-glycans in plants and acts at multiple stages of the plant N-glycosylation pathway, overview ?
-
?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein three potential N-glycosylation sites of the wild-type enzyme as well as of the recombinant enzyme are occupied by N-linked oligosaccharides, N-glycosylation is not essential for proper folding of XylT Arabidopsis thaliana
proteolytic modification proteolytic processing of recombinant XylT occurs between amino acids Arg178 and Arg179, a cleavage sequence compatible with the enzymatic properties of the baculoviral cysteine proteinase Arabidopsis thaliana

Reaction

Reaction Comment Organism Reaction ID
UDP-alpha-D-xylose + N4-[beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc]-L-asparaginyl-[protein] = UDP + N4-[beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->6)]-[beta-D-Xyl-(1->2)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc]-L-asparaginyl-[protein] the catalytic domain of XylT encompasses residues Lys57-Cys534 Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(Xylbeta1-2GnGn)-GP + UDP-xylose i.e. GnGnX Arabidopsis thaliana ?
-
?
GlcNAcbeta1-2Manalpha1-6(GlcNAcbeta1-2Manalpha1-3)Manbeta1-O-octyl + UDP-xylose i.e. GnGn-octyl Arabidopsis thaliana ?
-
?
additional information the enzyme is involved in the biosynthesis of glycoprotein-bound N-glycans in plants and acts at multiple stages of the plant N-glycosylation pathway, overview Arabidopsis thaliana ?
-
?
additional information the enzyme is specific for UDP-xylose, no activity with Man5-GP and MM-octyl, acceptor substrate specificity, overview Arabidopsis thaliana ?
-
?
Xylbeta1-2[Manalpha1-6(GlcNAcbeta1-2Manalpha1-3)]Manbeta1-O-octyl + UDP-xylose i.e. MGnX-octyl Arabidopsis thaliana ?
-
?
Xylbeta1-2[Manalpha1-6(Manalpha1-3)]Manbeta1-O-octyl + UDP-xylose i.e. MMX-octyl Arabidopsis thaliana ?
-
?
[GlcNAcbeta1-2Manalpha1-6(GlcNAcbeta1-2Manalpha1-3)Manbeta1-4GlcNAcbeta1-4(Fucalpha1-3)GlcNAc]-GP + UDP-xylose i.e. GnGnF Arabidopsis thaliana ?
-
?
[Manalpha1-6(Manalpha1-3)Manalpha1-6(GlcNAcbeta1-2Manalpha1-3)Manbeta1-4GlcNAcbeta1-4GlcNAc]-GP + UDP-xylose i.e. Man5Gn Arabidopsis thaliana ?
-
?

Subunits

Subunits Comment Organism
More primary sequence and hydrophobicity analysis of recombinant XylT Arabidopsis thaliana

Synonyms

Synonyms Comment Organism
beta1,2-xylosyltransferase
-
Arabidopsis thaliana
XylT
-
Arabidopsis thaliana

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Arabidopsis thaliana

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
the purified enzyme is stable for prolonged periods at neutral pH and at temperatures of up to 30°C Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 7 recombinant mutant DELTA39 XylT Arabidopsis thaliana

pH Stability

pH Stability pH Stability Maximum Comment Organism
additional information
-
the purified enzyme is stable for prolonged periods at neutral pH and at temperatures of up to 30°C Arabidopsis thaliana

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.042
-
UDP pH 7.0, 37°C, recombinant mutant DELTA39 XylT Arabidopsis thaliana
4.9
-
UDP-glucose pH 7.0, 37°C, recombinant mutant DELTA39 XylT Arabidopsis thaliana