Cloned (Comment) | Organism |
---|---|
expression of the enzyme's isolated CTS region, N-terminal 90 amino acids, and of the T and C region separately, as fusion protein with GFP in Nicotiana benthamiana leaf epidermal cells, the CT region, but not only the T or C regions, is sufficient to sustain Golgi retention without the involvement of any luminal sequences | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of transgenic Nicotiana benthamiana plants expressing the CTSregion, T and C region, and a membrane-shed enzyme version, the latter is secreted to the apoplast, the T or C region constructs are located in the cytoplasm, the CTS construct is located in the cytoplasmic membrane, overview | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasmic membrane | enzyme possesses a transmembrane domain | Arabidopsis thaliana | - |
- |
Golgi apparatus | the CTS region, formed by the N-terminal 90 amino acids, is responsible for correct targeting of the enzyme to the cytosplamic membrane | Arabidopsis thaliana | 5794 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-D-xylose + N4-[N-acetyl-beta-D-glucosaminyl-(1,2)-alpha-D-mannosyl-(1,3)-[N-acetyl-beta-D-glucosaminyl-(1,2)-alpha-D-mannosyl-(1,6)]-beta-D-mannosyl-(1,4)-N-acetyl-beta-D-glucosaminyl-(1,4)-N-acetyl-beta-D-glucosaminyl]asparagine | Arabidopsis thaliana | - |
UDP + N4-[N-acetyl-beta-D-glucosaminyl-(1,2)-alpha-D-mannosyl-(1,3)-[N-acetyl-beta-D-glucosaminyl-(1,2)-alpha-D-mannosyl-(1,6)]-[beta-D-xylosyl-(1,2)]-beta-D-mannosyl-(1,4)-N-acetyl-beta-D-glucosaminyl-(1,4)-N-acetyl-beta-D-glucosaminyl]asparagine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
- |
- |
Purification (Comment) | Organism |
---|---|
subcellular fractionation | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | transfer of D-xylose from UDP-D-xylose to the core beta-linked mannose of N-linked oligosaccharides | Arabidopsis thaliana | ? | - |
? | |
UDP-D-xylose + N4-[N-acetyl-beta-D-glucosaminyl-(1,2)-alpha-D-mannosyl-(1,3)-[N-acetyl-beta-D-glucosaminyl-(1,2)-alpha-D-mannosyl-(1,6)]-beta-D-mannosyl-(1,4)-N-acetyl-beta-D-glucosaminyl-(1,4)-N-acetyl-beta-D-glucosaminyl]asparagine | - |
Arabidopsis thaliana | UDP + N4-[N-acetyl-beta-D-glucosaminyl-(1,2)-alpha-D-mannosyl-(1,3)-[N-acetyl-beta-D-glucosaminyl-(1,2)-alpha-D-mannosyl-(1,6)]-[beta-D-xylosyl-(1,2)]-beta-D-mannosyl-(1,4)-N-acetyl-beta-D-glucosaminyl-(1,4)-N-acetyl-beta-D-glucosaminyl]asparagine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
beta1,2-xylosyltransferase | - |
Arabidopsis thaliana |
XylT | - |
Arabidopsis thaliana |