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Literature summary for 2.4.2.37 extracted from

  • Lowery, R.G.; Ludden, P.W.
    Effect of nucleotides on the activity of dinitrogenase reductase ADP-ribosyltransferase from Rhodospirillum rubrum (1989), Biochemistry, 28, 4956-4961.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
2'-deoxy-ADP activation Rhodospirillum rubrum
ADP-beta-S i.e. adenosine 5'-O-(2-thiodiphosphate), activation Rhodospirillum rubrum
MgADP- stimulation by binding to dinitrogen reductase (not from Azotobacter vinelandii), no activation by ATP, GDP, IDP, etheno-ADP, AMP-CH2-P, 8-bromo-ADP Rhodospirillum rubrum

General Stability

General Stability Organism
bovine serum albumin increases stability towards freeze thawing in the absence of ADP Rhodospirillum rubrum

Inhibitors

Inhibitors Comment Organism Structure
ATP in the presence of ADP, 63% inhibition at 1,5 mM Rhodospirillum rubrum
MgATP2- in the presence of Mg-ADP Rhodospirillum rubrum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.7
-
NAD+ cosubstrate ADP-D-ribosyl-[dinitrogen reductase] from Azotobacter vinelandii, in the absence of MgADP- Rhodospirillum rubrum
2
-
NAD+ cosubstrate native ADP-D-ribosyl-[dinitrogen reductase], in the presence of MgADP- Rhodospirillum rubrum
2.5
-
NAD+ cosubstrate ADP-D-ribosyl-[dinitrogen reductase] from Klebsiella pneumoniae, in the absence of MgADP- Rhodospirillum rubrum

Organism

Organism UniProt Comment Textmining
Rhodospirillum rubrum
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.004 0.015
-
Rhodospirillum rubrum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
NAD+ + [dinitrogen reductase] high specificity for acceptor substrate, the only acceptor is dinitrogen reductase Rhodospirillum rubrum nicotinamide + ADP-D-ribosyl-[dinitrogen reductase]
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Rhodospirillum rubrum

Cofactor

Cofactor Comment Organism Structure
2'-deoxy-ADP activation Rhodospirillum rubrum