Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2'-deoxy-ADP | activation | Rhodospirillum rubrum | |
ADP-beta-S | i.e. adenosine 5'-O-(2-thiodiphosphate), activation | Rhodospirillum rubrum | |
MgADP- | stimulation by binding to dinitrogen reductase (not from Azotobacter vinelandii), no activation by ATP, GDP, IDP, etheno-ADP, AMP-CH2-P, 8-bromo-ADP | Rhodospirillum rubrum |
General Stability | Organism |
---|---|
bovine serum albumin increases stability towards freeze thawing in the absence of ADP | Rhodospirillum rubrum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ATP | in the presence of ADP, 63% inhibition at 1,5 mM | Rhodospirillum rubrum | |
MgATP2- | in the presence of Mg-ADP | Rhodospirillum rubrum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.7 | - |
NAD+ | cosubstrate ADP-D-ribosyl-[dinitrogen reductase] from Azotobacter vinelandii, in the absence of MgADP- | Rhodospirillum rubrum | |
2 | - |
NAD+ | cosubstrate native ADP-D-ribosyl-[dinitrogen reductase], in the presence of MgADP- | Rhodospirillum rubrum | |
2.5 | - |
NAD+ | cosubstrate ADP-D-ribosyl-[dinitrogen reductase] from Klebsiella pneumoniae, in the absence of MgADP- | Rhodospirillum rubrum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodospirillum rubrum | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.004 | 0.015 | - |
Rhodospirillum rubrum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
NAD+ + [dinitrogen reductase] | high specificity for acceptor substrate, the only acceptor is dinitrogen reductase | Rhodospirillum rubrum | nicotinamide + ADP-D-ribosyl-[dinitrogen reductase] | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Rhodospirillum rubrum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
2'-deoxy-ADP | activation | Rhodospirillum rubrum |