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Literature summary for 2.4.2.36 extracted from

  • Fieldhouse, R.J.; Jurgensen, R.; Lugo, M.R.; Merrill, A.R.
    The 1.8 A cholix toxin crystal structure in complex with NAD+ and evidence for a new kinetic model (2012), J. Biol. Chem., 287, 21176-21188.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.4.2.36

Crystallization (Commentary)

Crystallization (Comment) Organism
a 1.8 A crystal structure of cholix in complex with its natural substrate, nicotinamide adenine dinucleotide Vibrio cholerae serotype O1

Protein Variants

Protein Variants Comment Organism
DELTA424-634 catalytic domain of cholix has much greater affinity for NAD+ than the full-length enzyme. 15fold higher ADP-ribosyltransferase activity than full-length enzyme Vibrio cholerae serotype O1
E574A mutation of catalytic fragment DELTA424-634: mutant does not have a large impact on NAD+-binding. Catalytic activity is 3fold reduced compared to catalytic fragment DELTA424-634 Vibrio cholerae serotype O1
E574A/E581A mutation of catalytic fragment DELTA424-634: mutant has low affinity for NAD+. Mutant shows no catalytic activity Vibrio cholerae serotype O1
E579R mutation of catalytic fragment DELTA424-634: NAD+ binding not changed significantly. Catalytic activity is 1.6fold reduced compared to catalytic fragment DELTA424-634 Vibrio cholerae serotype O1
E581A mutation of catalytic fragment DELTA424-634: mutant has low affinity for NAD+. Mutant shows no catalytic activity Vibrio cholerae serotype O1
Y493A mutation of catalytic fragment DELTA424-634: mutation of catalytic fragment DELTA424-634: mutant has little effect on NAD+-binding. Mutant shows a marked reduction in activity compared to catalytic fragment DELTA424-634 Vibrio cholerae serotype O1
Y504A mutation of catalytic fragment DELTA424-634: mutant has little effect on NAD+-binding. Mutant shows a marked reduction in activity compared to catalytic fragment DELTA424-634 Vibrio cholerae serotype O1
Y504F mutation of catalytic fragment DELTA424-634: mutant has little effect on NAD+-binding. Mutant has lower affinity for NAD+ than Y504A mutant. Mutant shows a mild reduction in activity compared to catalytic fragment DELTA424-634 Vibrio cholerae serotype O1

Inhibitors

Inhibitors Comment Organism Structure
4-amino-1,8-naphthalimide
-
 Vibrio cholerae serotype O1

Organism

Organism UniProt Comment Textmining
Vibrio cholerae serotype O1
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
NAD+ + elongation factor 2
-
 Vibrio cholerae serotype O1 nicotinamide + ADPribose-elongation factor 2
-
 ?

Synonyms

Synonyms Comment Organism
cholix
-
 Vibrio cholerae serotype O1

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Vibrio cholerae serotype O1

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.9
-
 assay at Vibrio cholerae serotype O1

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.042
-
 pH 7.9, 25°C Vibrio cholerae serotype O1 4-amino-1,8-naphthalimide