Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli LC1062 cells | Vibrio cholerae |
Protein Variants | Comment | Organism |
---|---|---|
E579Q | the mutant shows reduced biotinylation intensity compared to the wild type enzyme | Vibrio cholerae |
E581Q | the mutant exhibits strongly reduced biotinylation intensity compared to the wild type enzyme | Vibrio cholerae |
R426A | the mutant exhibits lower levels of NAD+ glycohydrolase activity than the wild type enzyme | Vibrio cholerae |
R426K/R451K/R473K/R479K/R506K/R519K/R522K/R525K/R535K/R540K/R543K/R566K/R629K | the mutant shows no auto-ADP-ribosylation activity and reduced NAD+ glycohydrolase activity compared to the wild type enzyme | Vibrio cholerae |
R426K/R451K/R473K/R479K/R506K/R519K/R522K/R535K/R540K/R543K/R566K/R629K | the mutant shows no auto-ADP-ribosylation activity and reduced NAD+ glycohydrolase activity compared to the wild type enzyme | Vibrio cholerae |
R426K/R451K/R473K/R506K/R519K/R522K/R525K/R535K/R540K/R543K/R566K/R629K | the mutant shows reduced auto-ADP-ribosylation activity and severely reduced NAD+ glycohydrolase activity compared to the wild type enzyme | Vibrio cholerae |
R426K/R451K/R473K/R506K/R519K/R522K/R535K/R540K/R543K/R566K/R629K | the mutant shows reduced auto-ADP-ribosylation activity and reduced NAD+ glycohydrolase activity compared to the wild type enzyme | Vibrio cholerae |
R426K/R451K/R473K/R506K/R519K/R522K/R540K/R543K/R566K/R629K | the mutant shows reduced auto-ADP-ribosylation activity and reduced NAD+ glycohydrolase activity compared to the wild type enzyme | Vibrio cholerae |
R426K/R473K/R479K/R506K/R519K/R522K/R566K/R629K | the mutant shows reduced auto-ADP-ribosylation activity and reduced NAD+ glycohydrolase activity compared to the wild type enzyme | Vibrio cholerae |
R426K/R473K/R506K/R519K/R522K/R540K/R543K/R566K/R629K | the mutant shows reduced auto-ADP-ribosylation activity and reduced NAD+ glycohydrolase activity compared to the wild type enzyme | Vibrio cholerae |
R426K/R473K/R506K/R519K/R522K/R540K/R566K/R629K | the mutant shows reduced auto-ADP-ribosylation activity and reduced NAD+ glycohydrolase activity compared to the wild type enzyme | Vibrio cholerae |
R426K/R473K/R506K/R519K/R522K/R543K/R566K/R629K | the mutant shows reduced auto-ADP-ribosylation activity and reduced NAD+ glycohydrolase activity compared to the wild type enzyme | Vibrio cholerae |
R426K/R473K/R506K/R519K/R522K/R566K/R629K | the mutant shows increased auto-ADP-ribosylation activity and reduced NAD+ glycohydrolase activity compared to the wild type enzyme | Vibrio cholerae |
R426K/R473K/R506K/R566K/R629K | the mutant shows reduced auto-ADP-ribosylation activity and reduced NAD+ glycohydrolase activity compared to the wild type enzyme | Vibrio cholerae |
R451A | the mutant exhibits lower levels of NAD+ glycohydrolase activity than the wild type enzyme | Vibrio cholerae |
R473A | the mutant exhibits lower levels of NAD+ glycohydrolase activity than the wild type enzyme | Vibrio cholerae |
R479A | the mutant exhibits lower levels of NAD+ glycohydrolase activity than the wild type enzyme | Vibrio cholerae |
R506A | the mutant exhibits lower levels of NAD+ glycohydrolase activity than the wild type enzyme | Vibrio cholerae |
R506K | the mutant exhibits lower levels of NAD+ glycohydrolase activity than the wild type enzyme | Vibrio cholerae |
R506K/R566K | the mutant shows reduced auto-ADP-ribosylation activity and reduced NAD+ glycohydrolase activity compared to the wild type enzyme | Vibrio cholerae |
R506Q | the mutant exhibits lower levels of NAD+ glycohydrolase activity than the wild type enzyme | Vibrio cholerae |
R519A | the mutant exhibits lower levels of NAD+ glycohydrolase activity than the wild type enzyme | Vibrio cholerae |
R522A | the mutant exhibits lower levels of NAD+ glycohydrolase activity than the wild type enzyme | Vibrio cholerae |
R525A | the mutant exhibits lower levels of NAD+ glycohydrolase activity than the wild type enzyme | Vibrio cholerae |
R525K | the mutant exhibits lower levels of NAD+ glycohydrolase activity than the wild type enzyme | Vibrio cholerae |
R525Q | the mutant exhibits lower levels of NAD+ glycohydrolase activity than the wild type enzyme | Vibrio cholerae |
R530A | the mutant exhibits lower levels of NAD+ glycohydrolase activity than the wild type enzyme | Vibrio cholerae |
R530K | inactive | Vibrio cholerae |
R530Q | inactive | Vibrio cholerae |
R535A | the mutant exhibits lower levels of NAD+ glycohydrolase activity than the wild type enzyme | Vibrio cholerae |
R540A | the mutant exhibits lower levels of NAD+ glycohydrolase activity than the wild type enzyme | Vibrio cholerae |
R543A | the mutant exhibits lower levels of NAD+ glycohydrolase activity than the wild type enzyme | Vibrio cholerae |
R566A | the mutant exhibits lower levels of NAD+ glycohydrolase activity than the wild type enzyme | Vibrio cholerae |
R629A | the mutant exhibits lower levels of NAD+ glycohydrolase activity than the wild type enzyme | Vibrio cholerae |
Y493A | inactive | Vibrio cholerae |
Y493A/E579Q/E581Q | inactive | Vibrio cholerae |
Y493A/E581Q | inactive | Vibrio cholerae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Vibrio cholerae | the enzyme also shows auto-ADP-ribosylation activity | ? | - |
? | |
NAD+ + protein L-arginine | Vibrio cholerae | - |
nicotinamide + Nomega-(ADP-D-ribosyl)-protein-L-arginine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Vibrio cholerae | Q5EK40 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography and Superdex 200 gel filtration | Vibrio cholerae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme also shows auto-ADP-ribosylation activity | Vibrio cholerae | ? | - |
? | |
NAD+ + eEF2 L-arginine | - |
Vibrio cholerae | nicotinamide + Nomega-(ADP-D-ribosyl)-eEF2-L-arginine | - |
? | |
NAD+ + protein L-arginine | - |
Vibrio cholerae | nicotinamide + Nomega-(ADP-D-ribosyl)-protein-L-arginine | - |
? | |
NAD+ + protein poly-L-arginine | - |
Vibrio cholerae | nicotinamide + Nomega-(ADP-D-ribosyl)-protein-poly-L-arginine | - |
? | |
NAD+ + protein R10H6 L-arginine | - |
Vibrio cholerae | nicotinamide + Nomega-(ADP-D-ribosyl)-protein N10H6-L-arginine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ADPRT | - |
Vibrio cholerae |
cholix toxin | - |
Vibrio cholerae |