Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Streptomyces scabiei |
Crystallization (Comment) | Organism |
---|---|
in complex with inhibitor PJ34 or P6, hanging drop vapor diffusion method, using 0.1 M MES, pH 6.5, and 15% (w/v) PEG 400 | Streptomyces scabiei |
Protein Variants | Comment | Organism |
---|---|---|
Q158A/E160A | the mutant shows reduced activity compared to the wild type enzyme | Streptomyces scabiei |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-[[3-(dimethylamino)-2-oxopropyl]amino]-5,6-dihydrophenanthridin-6-one | potent inhibitor | Streptomyces scabiei | |
4-[8-fluoro-6-oxo-1H,2H,3H,4H,5H,6H-benzo[c]1,6-naphthyridin-2-yl]butanoic acid | - |
Streptomyces scabiei | |
8-fluoro-2-[3-(piperidin-1-yl)propanesulfonyl]-1H,2H,3H,4H,5H,6H-benzo[c]1,6-naphthyridin-6-one | - |
Streptomyces scabiei | |
8-fluoro-2-[3-(piperidin-1-yl)propyl]-1H,2H,3H,4H,5H,6H-benzo[c]1,6-naphthyridin-6-one | - |
Streptomyces scabiei |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.068 | - |
NAD+ | wild type enzyme, at pH 7.9 and 22°C | Streptomyces scabiei |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
21962 | - |
x * 21962, MALDI-TOF mass spectrometry | Streptomyces scabiei |
22000 | - |
x * 22000, SDS-PAGE | Streptomyces scabiei |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
NAD+ + (ADP-D-ribosyl)n-acceptor | Streptomyces scabiei | the enzyme labels the exocyclic amino group on guanine bases in either single-stranded or double-stranded DNA | nicotinamide + (ADP-D-ribosyl)n+1-acceptor + H+ | - |
? | |
NAD+ + (ADP-D-ribosyl)n-acceptor | Streptomyces scabiei 87.22 | the enzyme labels the exocyclic amino group on guanine bases in either single-stranded or double-stranded DNA | nicotinamide + (ADP-D-ribosyl)n+1-acceptor + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces scabiei | C9Z6T8 | - |
- |
Streptomyces scabiei 87.22 | C9Z6T8 | - |
- |
Purification (Comment) | Organism |
---|---|
HiTrap chelating Sepharose column chromatography and HiTrap Q-Sepharose column chromatography | Streptomyces scabiei |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme also targets deoxyguanosine | Streptomyces scabiei | ? | - |
? | |
additional information | the enzyme also targets deoxyguanosine | Streptomyces scabiei 87.22 | ? | - |
? | |
NAD+ + (ADP-D-ribosyl)n-acceptor | the enzyme labels the exocyclic amino group on guanine bases in either single-stranded or double-stranded DNA | Streptomyces scabiei | nicotinamide + (ADP-D-ribosyl)n+1-acceptor + H+ | - |
? | |
NAD+ + (ADP-D-ribosyl)n-acceptor | the enzyme labels the exocyclic amino group on guanine bases in either single-stranded or double-stranded DNA | Streptomyces scabiei 87.22 | nicotinamide + (ADP-D-ribosyl)n+1-acceptor + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 22000, SDS-PAGE | Streptomyces scabiei |
? | x * 21962, MALDI-TOF mass spectrometry | Streptomyces scabiei |
Synonyms | Comment | Organism |
---|---|---|
ADP-ribosyltransferase | - |
Streptomyces scabiei |
scabin | - |
Streptomyces scabiei |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.005 | - |
NAD+ | mutant enzyme Q158A/E160A, at pH 7.9 and 22°C | Streptomyces scabiei | |
1.57 | - |
NAD+ | wild type enzyme, at pH 7.9 and 22°C | Streptomyces scabiei |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.003 | - |
2-[[3-(dimethylamino)-2-oxopropyl]amino]-5,6-dihydrophenanthridin-6-one | wild type enzyme, at pH 7.9 and 22°C | Streptomyces scabiei | |
0.007 | - |
8-fluoro-2-[3-(piperidin-1-yl)propanesulfonyl]-1H,2H,3H,4H,5H,6H-benzo[c]1,6-naphthyridin-6-one | wild type enzyme, at pH 7.9 and 22°C | Streptomyces scabiei | |
0.018 | - |
4-[8-fluoro-6-oxo-1H,2H,3H,4H,5H,6H-benzo[c]1,6-naphthyridin-2-yl]butanoic acid | wild type enzyme, at pH 7.9 and 22°C | Streptomyces scabiei | |
0.019 | - |
8-fluoro-2-[3-(piperidin-1-yl)propyl]-1H,2H,3H,4H,5H,6H-benzo[c]1,6-naphthyridin-6-one | wild type enzyme, at pH 7.9 and 22°C | Streptomyces scabiei | |
0.024 | - |
4-[8-fluoro-6-oxo-1H,2H,3H,4H,5H,6H-benzo[c]1,6-naphthyridin-2-yl]butanoic acid | wild type enzyme, at pH 7.9 and 22°C | Streptomyces scabiei |