Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 Codon Plus cells | Schistosoma mansoni |
Crystallization (Comment) | Organism |
---|---|
free isoform UPa and in complex with thymidine, uracil, thymine or 5-fluorouracil, sitting drop vapor diffusion method, using 100 mM trisodium citrate pH5.6, 16% (w/v) PEG4000 and 17.5% (v/v) isopropanol or 100 mM bis-Tris pH 5.5, 25% (w/v) PEG3350 and 200 mM ammonium sulfate | Schistosoma mansoni |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.031 | - |
uridine | pH and temperature not specified in the publication | Schistosoma mansoni | |
0.0489 | - |
thymidine | pH and temperature not specified in the publication | Schistosoma mansoni |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
uridine + phosphate | Schistosoma mansoni | - |
uracil + alpha-D-ribose 1-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Schistosoma mansoni | G4VGH9 | - |
- |
Purification (Comment) | Organism |
---|---|
Co-NTA agarose column chromatography | Schistosoma mansoni |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
thymidine + phosphate | - |
Schistosoma mansoni | thymine + 2-deoxy-alpha-D-ribose 1-phosphate | - |
? | |
uridine + phosphate | - |
Schistosoma mansoni | uracil + alpha-D-ribose 1-phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
uPA | isoform | Schistosoma mansoni |
UPb | inactive isoform | Schistosoma mansoni |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.41 | - |
thymidine | pH and temperature not specified in the publication | Schistosoma mansoni | |
35.9 | - |
uridine | pH and temperature not specified in the publication | Schistosoma mansoni |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
8.38 | - |
thymidine | pH and temperature not specified in the publication | Schistosoma mansoni | |
1158 | - |
uridine | pH and temperature not specified in the publication | Schistosoma mansoni |