Application | Comment | Organism |
---|---|---|
synthesis | the enzyme is a valuable industrial biocatalysts for high-temperature reactions that produce nucleoside drugs in high yields | Aeropyrum pernix |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli, the native apUP protein without fusion tags is actively expressed, and about 50% of the protein is found in the soluble fraction of the cell lysate | Aeropyrum pernix |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.248 | - |
5-methyluridine | pH 7.0, 90°C | Aeropyrum pernix | |
0.615 | - |
uridine | pH 7.0, 90°C | Aeropyrum pernix |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30200 | - |
6 * 30200, calculated from sequence | Aeropyrum pernix |
185300 | - |
gel filtration | Aeropyrum pernix |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aeropyrum pernix | Q9YA34 | - |
- |
Aeropyrum pernix DSM 11879 | Q9YA34 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Aeropyrum pernix |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-methyluridine + phosphate | 92% compared to the activity with uridine | Aeropyrum pernix | 5-methyluracil + alpha-D-ribose 1-phosphate | - |
? | |
5-methyluridine + phosphate | 92% compared to the activity with uridine | Aeropyrum pernix DSM 11879 | 5-methyluracil + alpha-D-ribose 1-phosphate | - |
? | |
deoxyuridine + phosphate | 66% compared to the activity with uridine | Aeropyrum pernix | uracil + deoxy-D-ribose 1-phosphate | - |
? | |
deoxyuridine + phosphate | 66% compared to the activity with uridine | Aeropyrum pernix DSM 11879 | uracil + deoxy-D-ribose 1-phosphate | - |
? | |
thymidine + phosphate | 60% compared to the activity with uridine | Aeropyrum pernix | thymine + 2-deoxy-alpha-D-ribose 1-phosphate | - |
? | |
thymidine + phosphate | 60% compared to the activity with uridine | Aeropyrum pernix DSM 11879 | thymine + 2-deoxy-alpha-D-ribose 1-phosphate | - |
? | |
uridine + phosphate | the optimal substrate for the enzyme is uridine. It shows no activity for 5-fluorouridine, cytidine, or 2-deoxycytidine | Aeropyrum pernix | uracil + alpha-D-ribose 1-phosphate | - |
? | |
uridine + phosphate | the optimal substrate for the enzyme is uridine. It shows no activity for 5-fluorouridine, cytidine, or 2-deoxycytidine | Aeropyrum pernix DSM 11879 | uracil + alpha-D-ribose 1-phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | 6 * 30200, calculated from sequence | Aeropyrum pernix |
Synonyms | Comment | Organism |
---|---|---|
apUP | - |
Aeropyrum pernix |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
- |
Aeropyrum pernix |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
after incubation for 60 min at 90 °C, the enzyme remains 100% active | Aeropyrum pernix |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
56 | - |
5-methyluridine | pH 7.0, 90°C | Aeropyrum pernix | |
150 | - |
uridine | pH 7.0, 90°C | Aeropyrum pernix |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Aeropyrum pernix |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3 | 9 | pH stability ranging from pH 3.0 to pH 9.0 | Aeropyrum pernix |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
230 | - |
5-methyluridine | pH 7.0, 90°C | Aeropyrum pernix | |
240 | - |
uridine | pH 7.0, 90°C | Aeropyrum pernix |