Literature summary for 2.4.2.3 extracted from

Lashkov, A.A.; Gabdoulkhakov, A.G.; Shtil, A.A.; Mikhailov, A.M.
Crystallization and preliminary X-ray c uridine phosphorylase complexed with 5-fluorouracil (2009), Acta Crystallogr. Sect. F, 65, 601-603.

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli strain BL21(DE3)	Salmonella enterica subsp. enterica serovar Typhimurium

Crystallization (Commentary)

Crystallization (Comment)	Organism
enzyme in complex with 5-fluorouracil, hanging-drop vapour-diffusion method, 0.5 ml of reservoir solution containing 0.34 ml 0.1 M Tris-maleate/NaOH buffer, pH 5.5, and 0.16 ml 40% w/v PEG 3350 is mixed with 2 ml protein solution containing 11.3 mg/ml enzyme in 10 mM Tris-HCl buffer, pH 7.3, 0.002 ml H2O, 0.0013 ml reservoir solution, 0.002 ml 100 mM 5-fluorouracil, and 0.0003 ml 2-propanol, 21°C, 1-2 weeks, X-ray diffraction structure determination and analysis at 2.2 A resolution, modeling	Salmonella enterica subsp. enterica serovar Typhimurium

Crystallization (Comment)

Organism

enzyme in complex with 5-fluorouracil, hanging-drop vapour-diffusion method, 0.5 ml of reservoir solution containing 0.34 ml 0.1 M Tris-maleate/NaOH buffer, pH 5.5, and 0.16 ml 40% w/v PEG 3350 is mixed with 2 ml protein solution containing 11.3 mg/ml enzyme in 10 mM Tris-HCl buffer, pH 7.3, 0.002 ml H2O, 0.0013 ml reservoir solution, 0.002 ml 100 mM 5-fluorouracil, and 0.0003 ml 2-propanol, 21°C, 1-2 weeks, X-ray diffraction structure determination and analysis at 2.2 A resolution, modeling

Salmonella enterica subsp. enterica serovar Typhimurium

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
additional information	Salmonella enterica subsp. enterica serovar Typhimurium	the enzyme also activates pyrimidine-containing drugs, including 5-fluorouracil, mechanism of the enzyme-drug interaction, overview	?	-	?
uridine + phosphate	Salmonella enterica subsp. enterica serovar Typhimurium	-	uracil + alpha-D-ribose 1-phosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Salmonella enterica subsp. enterica serovar Typhimurium	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant enzyme from Escherichia coli strain BL21(DE3) by hydrophobic interaction and anion exchange chromatography to 96% homogeneity	Salmonella enterica subsp. enterica serovar Typhimurium

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
additional information	the enzyme also activates pyrimidine-containing drugs, including 5-fluorouracil, mechanism of the enzyme-drug interaction, overview	Salmonella enterica subsp. enterica serovar Typhimurium	?	-	?
uridine + phosphate	-	Salmonella enterica subsp. enterica serovar Typhimurium	uracil + alpha-D-ribose 1-phosphate	-	?
uridine + phosphate	cleavage of the N-glycosidic bond	Salmonella enterica subsp. enterica serovar Typhimurium	uracil + alpha-D-ribose 1-phosphate	-	?

Synonyms

Synonyms	Comment	Organism
UPH	-	Salmonella enterica subsp. enterica serovar Typhimurium