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Literature summary for 2.4.2.3 extracted from

  • Bu, W.; Settembre, E.C.; el Kouni, M.H.; Ealick, S.E.
    Structural basis for inhibition of Escherichia coli uridine phosphorylase by 5-substituted acyclouridines (2005), Acta Crystallogr. Sect. D, 61, 863-872.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging-drop vapour-diffusion method, structures of 5-benzylacyclouridine, 5-phenylthioacyclouridine, 5-phenylselenylacyclouridine, 5-m-benzyloxybenzyl acyclouridine and 5-m-benzyloxybenzyl barbituric acid acyclonucleoside bound to the active site of the enzyme, resolutions ranging from 1.95 to 2.3 A Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
5-benzylacyclouridine
-
Escherichia coli
5-m-benzyloxybenzyl acyclouridine
-
Escherichia coli
5-m-benzyloxybenzyl barbituric acid acyclonucleoside
-
Escherichia coli
5-phenylselenenylacyclouridine
-
Escherichia coli
5-phenylthioacyclouridine
-
Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
uridine + phosphate Escherichia coli key enzyme in pyrimidine-salvage pathway uracil + alpha-D-ribose 1-phosphate
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli P12758
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
uridine + phosphate
-
Escherichia coli uracil + alpha-D-ribose 1-phosphate
-
?
uridine + phosphate key enzyme in pyrimidine-salvage pathway Escherichia coli uracil + alpha-D-ribose 1-phosphate
-
?

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00068
-
5-m-benzyloxybenzyl acyclouridine
-
Escherichia coli
0.0043
-
5-benzylacyclouridine
-
Escherichia coli