Crystallization (Comment) | Organism |
---|---|
wild-type at pH 5.5 to 1.9 A resolution and in complex with pre-queuine 0 to 1.7 A resolution (both crystals belong to space group C2), and in complex with its natural substrate pre-queuine 1 to 2.4 A resolution (crystal belongs to space group C2221). Mutant Y106F alone to 1.95 A resolution and in complex with pre-queuine 1 to 1.9 A resolution (both crystals belong to space group C2). By macroseeding and the hanging-drop method | Zymomonas mobilis |
Protein Variants | Comment | Organism |
---|---|---|
Y106F | crystallises similarly to the wild-type | Zymomonas mobilis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
6-amino-2-(methylamino)-3-[(methylamino)methyl]-3,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one | - |
Zymomonas mobilis | |
6-amino-2-(methylamino)-3-[2-(methylamino)ethyl]-3,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one | - |
Zymomonas mobilis | |
6-amino-2-(methylamino)-4-[(methylamino)methyl]-1,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one | - |
Zymomonas mobilis | |
6-amino-2-(methylamino)-4-[2-(methylamino)ethyl]-1,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one | - |
Zymomonas mobilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00036 | - |
[tRNA]-guanine | mutant Y106F | Zymomonas mobilis | |
0.00038 | - |
guanine | wild-type | Zymomonas mobilis | |
0.001 | - |
[tRNA]-guanine | wild-type | Zymomonas mobilis | |
0.0013 | - |
guanine | mutant Y106F | Zymomonas mobilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Zymomonas mobilis | P28720 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
7-aminomethyl-7-carbaguanine + [tRNA]-guanine | Tyr106 in the active site is hydrogen bonded in the ligand-free structure | Zymomonas mobilis | guanine + [tRNA]-pre Q1 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
TGT | - |
Zymomonas mobilis |
tRNA-guanine transglycosylase | - |
Zymomonas mobilis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.004 | - |
guanine | mutant Y106F | Zymomonas mobilis | |
0.005 | - |
[tRNA]-guanine | mutant Y106F | Zymomonas mobilis | |
0.011 | - |
guanine | wild-type | Zymomonas mobilis | |
0.014 | - |
[tRNA]-guanine | wild-type | Zymomonas mobilis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.4 | - |
guanine | mutant Y106F | Zymomonas mobilis | |
13.3 | - |
[tRNA]-guanine | mutant Y106F | Zymomonas mobilis | |
13.9 | - |
[tRNA]-guanine | wild-type | Zymomonas mobilis | |
28.9 | - |
guanine | wild-type | Zymomonas mobilis |