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Literature summary for 2.4.2.29 extracted from

  • Ritschel, T.; Kohler, P.C.; Neudert, G.; Heine, A.; Diederich, F.; Klebe, G.
    How to replace the residual solvation shell of polar active site residues to achieve nanomolar inhibition of tRNA-guanine transglycosylase (2009), ChemMedChem, 4, 2012-2023.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
wild-type at pH 5.5 to 1.9 A resolution and in complex with pre-queuine 0 to 1.7 A resolution (both crystals belong to space group C2), and in complex with its natural substrate pre-queuine 1 to 2.4 A resolution (crystal belongs to space group C2221). Mutant Y106F alone to 1.95 A resolution and in complex with pre-queuine 1 to 1.9 A resolution (both crystals belong to space group C2). By macroseeding and the hanging-drop method Zymomonas mobilis

Protein Variants

Protein Variants Comment Organism
Y106F crystallises similarly to the wild-type Zymomonas mobilis

Inhibitors

Inhibitors Comment Organism Structure
6-amino-2-(methylamino)-3-[(methylamino)methyl]-3,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one
-
Zymomonas mobilis
6-amino-2-(methylamino)-3-[2-(methylamino)ethyl]-3,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one
-
Zymomonas mobilis
6-amino-2-(methylamino)-4-[(methylamino)methyl]-1,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one
-
Zymomonas mobilis
6-amino-2-(methylamino)-4-[2-(methylamino)ethyl]-1,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one
-
Zymomonas mobilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00036
-
[tRNA]-guanine mutant Y106F Zymomonas mobilis
0.00038
-
guanine wild-type Zymomonas mobilis
0.001
-
[tRNA]-guanine wild-type Zymomonas mobilis
0.0013
-
guanine mutant Y106F Zymomonas mobilis

Organism

Organism UniProt Comment Textmining
Zymomonas mobilis P28720
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7-aminomethyl-7-carbaguanine + [tRNA]-guanine Tyr106 in the active site is hydrogen bonded in the ligand-free structure Zymomonas mobilis guanine + [tRNA]-pre Q1
-
?

Synonyms

Synonyms Comment Organism
TGT
-
Zymomonas mobilis
tRNA-guanine transglycosylase
-
Zymomonas mobilis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.004
-
guanine mutant Y106F Zymomonas mobilis
0.005
-
[tRNA]-guanine mutant Y106F Zymomonas mobilis
0.011
-
guanine wild-type Zymomonas mobilis
0.014
-
[tRNA]-guanine wild-type Zymomonas mobilis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.4
-
guanine mutant Y106F Zymomonas mobilis
13.3
-
[tRNA]-guanine mutant Y106F Zymomonas mobilis
13.9
-
[tRNA]-guanine wild-type Zymomonas mobilis
28.9
-
guanine wild-type Zymomonas mobilis