Literature summary for 2.4.2.28 extracted from

Bartasun, P.; Cieslinski, H.; Bujacz, A.; Wierzbicka-Wos, A.; Kur, J.
A study on the interaction of rhodamine B with methylthioadenosine phosphorylase protein sourced from an antarctic soil metagenomic library (2013), PLoS ONE, 8, e55697.

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3)pLysS cells	uncultured bacterium

Crystallization (Commentary)

Crystallization (Comment)	Organism
apoenzyme and in complex with rhodamine B, hanging drop vapor diffusion method, using 35% (w/v) PPG 400, 75 mM MgCl2 and 0.1 M Bis-Tris pH 6.5	uncultured bacterium

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
30000	-	3 * 30000, SDS-PAGE	uncultured bacterium
32000	-	3 * 32000, calculated from amino acid sequence	uncultured bacterium
97000	-	gel filtration	uncultured bacterium

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
S-methyl-5'-thioadenosine + phosphate	uncultured bacterium	-	adenine + S-methyl-5-thio-alpha-D-ribose 1-phosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
uncultured bacterium	C6KFA4	-	-

Purification (Commentary)

Purification (Comment)	Organism
Fractogel EMD DEAE column chromatograph and Resource Q column chromatography	uncultured bacterium

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
S-methyl-5'-thioadenosine + phosphate	-	uncultured bacterium	adenine + S-methyl-5-thio-alpha-D-ribose 1-phosphate	-	?

Subunits

Subunits	Comment	Organism
homotrimer	3 * 30000, SDS-PAGE	uncultured bacterium
homotrimer	3 * 32000, calculated from amino acid sequence	uncultured bacterium

Synonyms

Synonyms	Comment	Organism
methylthioadenosine phosphorylase	-	uncultured bacterium
RSFP	-	uncultured bacterium

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Release 2023.1 (January 2023)