Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Mycobacterium tuberculosis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5'-methylthio-DADMe-immucillin A | - |
Mycobacterium tuberculosis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.009 | - |
5'-methylthioadenosine | pH and temperature not specified in the publication, co-substrate: 50 mM phosphate | Mycobacterium tuberculosis | |
0.26 | - |
phosphate | pH and temperature not specified in the publication, co-substrate: 0.2 mM 5'-methylthioadenosine | Mycobacterium tuberculosis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30000 | - |
SDS-PAGE | Mycobacterium tuberculosis |
60000 | - |
gel filtration | Mycobacterium tuberculosis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | O06401 | - |
- |
Storage Stability | Organism |
---|---|
-20°C, stable | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5'-methylthioadenosine + phosphate | - |
Mycobacterium tuberculosis | adenine + S-methyl-5-thio-alpha-D-ribose 1-phosphate | - |
? | |
adenosine + phosphate | 2.1% activity compared to activity with 5'-methylthioadenosine | Mycobacterium tuberculosis | adenine + D-ribose 1-phosphate | - |
? | |
S-adenosyl-L-homocysteine + phosphate | 0.81% activity compared to activity with 5'-methylthioadenosine | Mycobacterium tuberculosis | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 30000, SDS-PAGE | Mycobacterium tuberculosis |
Synonyms | Comment | Organism |
---|---|---|
MTAP | - |
Mycobacterium tuberculosis |
Rv0535 | - |
Mycobacterium tuberculosis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
4 | - |
4h, 40% loss of activity | Mycobacterium tuberculosis |
37 | - |
4h, 90% loss of activity | Mycobacterium tuberculosis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.26 | - |
phosphate | pH and temperature not specified in the publication, co-substrate: 0.2 mM 5'-methylthioadenosine | Mycobacterium tuberculosis | |
0.4 | - |
5'-methylthioadenosine | pH and temperature not specified in the publication, co-substrate: 50 mM phosphate | Mycobacterium tuberculosis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
7-7.5 optimal pH | Mycobacterium tuberculosis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 9 | pH 6.5: about 55% of maximal activity, pH 9.0: about 55% of maximal activity | Mycobacterium tuberculosis |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.000025 | - |
pH and temperature not specified in the publication, in the presence of 10 microM 5'-methylthioadenosine | Mycobacterium tuberculosis | 5'-methylthio-DADMe-immucillin A | |
0.000075 | - |
pH and temperature not specified in the publication, in the presence of 100 microM 5'-methylthioadenosine | Mycobacterium tuberculosis | 5'-methylthio-DADMe-immucillin A |