Application | Comment | Organism |
---|---|---|
medicine | gene is linked to the important tumor suppressor gene p16INK4A, enzyme is of key importance in defining the chromosomal area homozygously deleted in a large number of human tumors, absence of enzyme activity only in malignant cells is of great value in developing selective antitumor therapeutic strategies | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
the gene maps on the 9p21 chromosome, strictly linked to the tumor suppressor gene p16INK4A, functional expression in Escherichia coli strain JM105 | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5'-Chloroformycin | - |
Homo sapiens | |
5'-methylthiotubercidin | - |
Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.004 | - |
5'-methylthioadenosine | recombinant enzyme | Homo sapiens | |
0.75 | - |
phosphate | recombinant enzyme | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30000 | - |
3 * 30000, recombinant enzyme, SDS-PAGE | Homo sapiens |
90000 | - |
recombinant enzyme, gel filtration | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant from Escherichia coli | Homo sapiens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
9.5 | - |
purified recombinant enzyme | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5'-methylthioadenosine + phosphate | - |
Homo sapiens | adenine + 5-methylthio-D-ribose 1-phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
trimer | 3 * 30000, recombinant enzyme, SDS-PAGE | Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Homo sapiens |