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Literature summary for 2.4.2.26 extracted from
- Heterologous expression and biochemical characterization of soluble human xylosyltransferase II (2008), Biochem. Biophys. Res. Commun., 365, 678-684.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | 84% activation at 25 mM | Homo sapiens |  |
| histone protein | activity gains 1.5fold when histone protein is added at a concentration of 0.1 mg/ml | Homo sapiens | |
| protamine | adding protamine to a final concentration of 0.1 mg/ml stimulates XT-II activity at least 2fold | Homo sapiens | |
| Sodium citrate | 72% activation at 25 mM | Homo sapiens | |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Pichia pastoris strain X-33 | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| beta-mercaptoethanol | XT-II activity is completely abolished at a concentration 1% (v/v) beta-mercaptoethanol | Homo sapiens | |
| CDP | 23% residual activity at 10 mg/ml | Homo sapiens | |
| CMP | 45% residual activity at 10 mg/ml | Homo sapiens | |
| Co2+ | Co2+ reduces activity by more than 60% at 5 mM | Homo sapiens | |
| CTP | 8% residual activity at 10 mg/ml | Homo sapiens | |
| Cu2+ | complete inhibition at 5 mM | Homo sapiens | |
| dithiothreitol | XT-II activity is completely abolished at a concentration of 1 mM dithiothreitol | Homo sapiens | |
| additional information | lysozyme does not change the activity of XT-II at any concentration | Homo sapiens | |
| Ni2+ | complete inhibition at 5 mM | Homo sapiens | |
| protamine | adding protamine to a final concentration of 10 mg/ml decreases XT-II activity 4fold | Homo sapiens | |
| UDP | 5% residual activity at 10 mg/ml | Homo sapiens | |
| UMP | 25% residual activity at 10 mg/ml | Homo sapiens | |
| UTP | 4% residual activity at 10 mg/ml | Homo sapiens | |
| Zn2+ | complete inhibition at 5 mM | Homo sapiens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0019 | - |
Bio-QEEEGSGGGQKK-F | - |
Homo sapiens | |
| 0.0108 | - |
bikunin | - |
Homo sapiens | |
| 0.0191 | - |
fibroblast growth factor 2 fragment 1-24 | - |
Homo sapiens |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | necessity for Mg2+ or Mn2+ ions for enzymatic activity | Homo sapiens | |
| Mn2+ | necessity for Mg2+ or Mn2+ ions for enzymatic activity | Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 110000 | - |
gel filtration | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | Q9H1B5 | - |
- |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -80°C, -20°C or 4°C, 3-4 weeks, no loss of activity | Homo sapiens |
| 25°C, 2 weeks, no loss of activity | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | TENEGSGLTNIK, SENEGSGMAEQK, orcokinin (NFDEIDRSGFGFN), melittin (GIGAVLKVLTTGLPALISWIKRKRQQ), fibrinopeptide A (ADSGEGDFLAEGGGVR), IgE C4 domain (KTKGSGFFVF), C-type natriuretic peptide (GLSKGCFGLKLDRIGSMSGLGC), and calcitonin (VLGKLSQELHKLQTYPRTNTGSGTP) are no substrates for XT-II | Homo sapiens | ? | - |
? | |
| UDP-D-xylose + bikunin | - |
Homo sapiens | UDP + D-xylosyl-bikunin | - |
? | |
| UDP-D-xylose + biotin-NH-QEEEGSGGGQKK(5-fluorescein)-CONH2 | the bikunin-homologous peptide Bio-QEEEGSGGGQKK-F is best acceptor substrate for XT-II, 100% activity with UDP-D-xylose | Homo sapiens | UDP + biotin-NH-QEEEG-(D-xylosyl)SGGGQKK(5-fluorescein)-CONH2 | - |
? | |
| UDP-D-xylose + fibroblast growth factor 2 fragment 1-24 | - |
Homo sapiens | ? | - |
? | |
| UDP-D-xylose + silk fibroin | - |
Homo sapiens | UDP + silk fibroin with xylosylserine | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| XT-II | - |
Homo sapiens |
| xylosyltransferase II | isoform of xylosyltransferase I | Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
- |
Homo sapiens |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 4 | 45 | - |
Homo sapiens |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
reaching a temperature of 37°C, XT-II has 66% of the activity compared with 30°C | Homo sapiens |
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