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Literature summary for 2.4.2.26 extracted from

  • Pfeil, U.; Wenzel, K.W.
    Purification and some properties of UDP-xylosyltransferase of rat ear cartilage (2000), Glycobiology, 10, 803-807.
    View publication on PubMed
Search for more literature for 2.4.2.26

Inhibitors

Inhibitors Comment Organism Structure
Zn2+ strong Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information
-
 Rattus norvegicus
0.0065
-
 UDP-D-xylose
-
 Rattus norvegicus
0.008
-
 QEEEGSGGGQGG
-
 Rattus norvegicus
0.093
-
 QEEEGSGGGQKK
-
 Rattus norvegicus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ essentially required, Mg2+ and Mn2+ are equally effective Rattus norvegicus
Mg2+ essentially required, Ca2+ and Mn2+ are equally effective Rattus norvegicus
Mn2+ essentially required, Mg2+ and Ca2+ are equally effective Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
71000
-
 gel filtration Rattus norvegicus
78000
-
 1 * 78000, SDS-PAGE Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
affinity chromatography on QEEEGSGGGQGG-resin Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
cartilage from ear Rattus norvegicus
-
ear cartilage Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.419
-
 purified enzyme Rattus norvegicus

Storage Stability

Storage Stability Organism
-20°C or -80°C, Tris-HCl 50 mM, pH 7.0, NaCl 50 mM, stable for at least 15 weeks Rattus norvegicus
storage at 4°C, 25°C or 37°C lead to rapid loss of activity Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information substrate specificity Rattus norvegicus ?
-
 ?
UDP-alpha-D-xylose + [protein]-L-serine transfers a beta-D-xylosyl residue from UDP-D-xylose to the serine hydroxyl group of an acceptor protein substrate Rattus norvegicus UDP + [protein]-3-O-(beta-D-xylosyl)-L-serine
-
 ?
UDP-alpha-D-xylose + [protein]-L-serine transfers a beta-D-xylosyl residue to specific serine residues dependent on the consensus signal sequence Rattus norvegicus UDP + [protein]-3-O-(beta-D-xylosyl)-L-serine
-
 ?
UDP-D-xylose + QEEEGSGGGQGG peptide derived from bikunin Rattus norvegicus UDP + QEEEGS(-D-xylose)GGGQGG
-
 ?
UDP-D-xylose + QEEEGSGGGQKK peptide derived from bikunin Rattus norvegicus UDP + QEEEGS(-D-xylose)GGGQKK
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 78000, SDS-PAGE Rattus norvegicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 depends on buffer system Rattus norvegicus