General Stability | Organism |
---|---|
repeated cycles of freezing and thawing inactivate the enzyme if phosphate is absent | Haemophilus influenzae |
the enzyme is quite labile during the course of purification, dithiothreitol and glycerol are required for stability | Haemophilus influenzae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | - |
5-Bromouridine | - |
Haemophilus influenzae | |
0.07 | - |
5-methyluridine | - |
Haemophilus influenzae | |
0.1 | - |
5-bromodeoxyuridine | - |
Haemophilus influenzae | |
0.11 | - |
thymidine | - |
Haemophilus influenzae | |
0.13 | - |
deoxyuridine | - |
Haemophilus influenzae | |
0.24 | - |
uridine | - |
Haemophilus influenzae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Haemophilus influenzae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Haemophilus influenzae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
25.5 | - |
- |
Haemophilus influenzae |
Storage Stability | Organism |
---|---|
-20°C, in presence of phosphate, glycerol and dithiothreitol, stable for 6 months | Haemophilus influenzae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-bromodeoxyuridine + phosphate | 74% of the activity with uridine | Haemophilus influenzae | 5-bromouracil + deoxyribose 1-phosphate | - |
? | |
5-bromouridine + phosphate | 40% of the activity with uridine | Haemophilus influenzae | 5-bromouracil + alpha-D-ribose 1-phosphate | - |
? | |
5-methyluridine + phosphate | 27% of the activity with uridine | Haemophilus influenzae | 5-methyluracil + alpha-D-ribose 1-phosphate | - |
? | |
deoxyuridine + phosphate | 12% of the activity with uridine | Haemophilus influenzae | uracil + 2-deoxy-D-ribose 1-phosphate | - |
? | |
thymidine + phosphate | 21% of the activity with uridine | Haemophilus influenzae | thymine + 2-deoxy-D-ribose 1-phosphate | - |
? | |
uracil arabinoside + phosphate | - |
Haemophilus influenzae | uracil + alpha-D-arabinose 1-phosphate | - |
? | |
uridine + phosphate | - |
Haemophilus influenzae | uracil + alpha-D-ribose 1-phosphate | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.9 | - |
- |
Haemophilus influenzae |
7.4 | - |
ribonucleosides and uracil arabinoside | Haemophilus influenzae |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.9 | 7.4 | pH 6.9: optimum, pH 7.4: 75% of maximal activity | Haemophilus influenzae |