Cloned (Comment) | Organism |
---|---|
gene TM1645, expression of N-terminally His-tagged enzyme in Escherichia coli | Thermotoga maritima |
Crystallization (Comment) | Organism |
---|---|
purified recombinant enzyme, hanging drop vapour diffusion method, 10 mg/ml protein, crystallization solution contains 10% PEG 6000, and 0.1 M MES, pH 6.0, X-ray diffraction structure determination and analysis at 2.5 A resolution, molecular replacement, model construction | Thermotoga maritima |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30048 | - |
x * 30048, crystal structure, residues 1-273 | Thermotoga maritima |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
pyridine-2,3-dicarboxylate + 5-phospho-alpha-D-ribose 1-diphosphate | Thermotoga maritima | enzyme provides the de novo source of nicotinate mononucleotide | nicotinate D-ribonucleotide + diphosphate + CO2 | - |
? | |
pyridine-2,3-dicarboxylate + 5-phospho-alpha-D-ribose 1-diphosphate | Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 | enzyme provides the de novo source of nicotinate mononucleotide | nicotinate D-ribonucleotide + diphosphate + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermotoga maritima | Q9X1X8 | gene TM1645 | - |
Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 | Q9X1X8 | gene TM1645 | - |
Purification (Comment) | Organism |
---|---|
recombinant N-terminally His-tagged enzyme from Escherichia coli b nickel affinity chromatography | Thermotoga maritima |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
pyridine-2,3-dicarboxylate + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Thermotoga maritima | nicotinate D-ribonucleotide + diphosphate + CO2 | - |
? | |
pyridine-2,3-dicarboxylate + 5-phospho-alpha-D-ribose 1-diphosphate | enzyme provides the de novo source of nicotinate mononucleotide | Thermotoga maritima | nicotinate D-ribonucleotide + diphosphate + CO2 | - |
? | |
pyridine-2,3-dicarboxylate + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 | nicotinate D-ribonucleotide + diphosphate + CO2 | - |
? | |
pyridine-2,3-dicarboxylate + 5-phospho-alpha-D-ribose 1-diphosphate | enzyme provides the de novo source of nicotinate mononucleotide | Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 | nicotinate D-ribonucleotide + diphosphate + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 30048, crystal structure, residues 1-273 | Thermotoga maritima |
Synonyms | Comment | Organism |
---|---|---|
NadC | - |
Thermotoga maritima |
QAPRTase | - |
Thermotoga maritima |
TM1645 | - |
Thermotoga maritima |
type II quinolic acid phosphoribosyltransferase | - |
Thermotoga maritima |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Thermotoga maritima | sequence calculation, residues 1-273 | - |
5.1 |