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Literature summary for 2.4.2.19 extracted from

  • Schwarzenbacher, R.; Jaroszewski, L.; Von Delft, F.; Abdubek, P.; Ambing, E.; Biorac, T.; Brinen, L.S.; Canaves, J.M.; Cambell, J.; Chiu, H.J.; et al.
    Crystal structure of a type II quinolic acid phosphoribosyltransferase (TM1645) from Thermotoga maritima at 2.50 A resolution (2004), Proteins Struct. Funct. Bioinform., 55, 768-771.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene TM1645, expression of N-terminally His-tagged enzyme in Escherichia coli Thermotoga maritima

Crystallization (Commentary)

Crystallization (Comment) Organism
purified recombinant enzyme, hanging drop vapour diffusion method, 10 mg/ml protein, crystallization solution contains 10% PEG 6000, and 0.1 M MES, pH 6.0, X-ray diffraction structure determination and analysis at 2.5 A resolution, molecular replacement, model construction Thermotoga maritima

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30048
-
x * 30048, crystal structure, residues 1-273 Thermotoga maritima

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
pyridine-2,3-dicarboxylate + 5-phospho-alpha-D-ribose 1-diphosphate Thermotoga maritima enzyme provides the de novo source of nicotinate mononucleotide nicotinate D-ribonucleotide + diphosphate + CO2
-
?
pyridine-2,3-dicarboxylate + 5-phospho-alpha-D-ribose 1-diphosphate Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 enzyme provides the de novo source of nicotinate mononucleotide nicotinate D-ribonucleotide + diphosphate + CO2
-
?

Organism

Organism UniProt Comment Textmining
Thermotoga maritima Q9X1X8 gene TM1645
-
Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 Q9X1X8 gene TM1645
-

Purification (Commentary)

Purification (Comment) Organism
recombinant N-terminally His-tagged enzyme from Escherichia coli b nickel affinity chromatography Thermotoga maritima

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
pyridine-2,3-dicarboxylate + 5-phospho-alpha-D-ribose 1-diphosphate
-
Thermotoga maritima nicotinate D-ribonucleotide + diphosphate + CO2
-
?
pyridine-2,3-dicarboxylate + 5-phospho-alpha-D-ribose 1-diphosphate enzyme provides the de novo source of nicotinate mononucleotide Thermotoga maritima nicotinate D-ribonucleotide + diphosphate + CO2
-
?
pyridine-2,3-dicarboxylate + 5-phospho-alpha-D-ribose 1-diphosphate
-
Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 nicotinate D-ribonucleotide + diphosphate + CO2
-
?
pyridine-2,3-dicarboxylate + 5-phospho-alpha-D-ribose 1-diphosphate enzyme provides the de novo source of nicotinate mononucleotide Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 nicotinate D-ribonucleotide + diphosphate + CO2
-
?

Subunits

Subunits Comment Organism
? x * 30048, crystal structure, residues 1-273 Thermotoga maritima

Synonyms

Synonyms Comment Organism
NadC
-
Thermotoga maritima
QAPRTase
-
Thermotoga maritima
TM1645
-
Thermotoga maritima
type II quinolic acid phosphoribosyltransferase
-
Thermotoga maritima

pI Value

Organism Comment pI Value Maximum pI Value
Thermotoga maritima sequence calculation, residues 1-273
-
5.1