Cloned (Comment) | Organism |
---|---|
- |
Saccharolobus solfataricus |
expression in Escherichia coli | Saccharolobus solfataricus |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method | Saccharolobus solfataricus |
hanging-drop method, crystals of ssTrpD diffract to better than 2.6 A resolution | Saccharolobus solfataricus |
General Stability | Organism |
---|---|
the enzyme is either digested by trypsin, V8-protease or thermolysin after incubation for 1 h at 25°C but not by thermolysin at 70°C | Saccharolobus solfataricus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000005 | - |
anthranilate | at 87°C, Km decreases at lower temperatures | Saccharolobus solfataricus | |
0.002 | - |
5-phospho-alpha-D-ribose 1-diphosphate | 25°C | Saccharolobus solfataricus | |
0.002 | - |
anthranilate | 25°C | Saccharolobus solfataricus | |
0.0026 | - |
5-phospho-alpha-D-ribose 1-diphosphate | 40°C | Saccharolobus solfataricus | |
0.0026 | - |
anthranilate | 40°C | Saccharolobus solfataricus | |
0.0032 | - |
5-phospho-alpha-D-ribose 1-diphosphate | 50°C | Saccharolobus solfataricus | |
0.0032 | - |
anthranilate | 50°C | Saccharolobus solfataricus | |
0.0035 | - |
5-phospho-alpha-D-ribose 1-diphosphate | 60°C | Saccharolobus solfataricus | |
0.0035 | - |
anthranilate | 60°C | Saccharolobus solfataricus | |
0.005 | - |
5-phospho-alpha-D-ribose 1-diphosphate | 87°C | Saccharolobus solfataricus | |
0.005 | - |
anthranilate | 87°C | Saccharolobus solfataricus | |
0.01 | - |
5-phospho-alpha-D-ribose 1-diphosphate | - |
Saccharolobus solfataricus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
73300 | - |
gel filtration | Saccharolobus solfataricus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
anthranilate + 5-phospho-alpha-D-ribose 1-diphosphate | Saccharolobus solfataricus | enzyme of tryptophan biosynthesis | N-(5-phospho-D-ribosyl)-anthranilate + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus solfataricus | - |
- |
- |
Saccharolobus solfataricus | P50384 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Saccharolobus solfataricus |
95% pure | Saccharolobus solfataricus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
anthranilate + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Saccharolobus solfataricus | N-(5-phospho-D-ribosyl)-anthranilate + diphosphate | - |
? | |
anthranilate + 5-phospho-alpha-D-ribose 1-diphosphate | enzyme of tryptophan biosynthesis | Saccharolobus solfataricus | N-(5-phospho-D-ribosyl)-anthranilate + diphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | - |
Saccharolobus solfataricus |
Synonyms | Comment | Organism |
---|---|---|
ssTrpD | - |
Saccharolobus solfataricus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
50% loss of activity after 69 min | Saccharolobus solfataricus |
70 | - |
t1/2: 69 min, addition of anthranilate has no stabilizing effect | Saccharolobus solfataricus |
85 | - |
50% loss of activity after 35 min | Saccharolobus solfataricus |
85 | - |
t1/2: 35 min, addition of anthranilate has no stabilizing effect | Saccharolobus solfataricus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.014 | - |
5-phospho-alpha-D-ribose 1-diphosphate | 25°C | Saccharolobus solfataricus | |
0.014 | - |
anthranilate | 25°C | Saccharolobus solfataricus | |
0.014 | - |
anthranilate | at 25°C | Saccharolobus solfataricus | |
0.035 | - |
anthranilate | at 40°C | Saccharolobus solfataricus | |
0.035 | - |
5-phospho-alpha-D-ribose 1-diphosphate | 40°C | Saccharolobus solfataricus | |
0.035 | - |
anthranilate | 40°C | Saccharolobus solfataricus | |
0.067 | - |
anthranilate | at 50°C | Saccharolobus solfataricus | |
0.067 | - |
5-phospho-alpha-D-ribose 1-diphosphate | 50°C | Saccharolobus solfataricus | |
0.067 | - |
anthranilate | 50°C | Saccharolobus solfataricus | |
0.105 | - |
5-phospho-alpha-D-ribose 1-diphosphate | 60°C | Saccharolobus solfataricus | |
0.105 | - |
anthranilate | 60°C | Saccharolobus solfataricus | |
0.105 | - |
anthranilate | at 60°C | Saccharolobus solfataricus | |
0.4 | - |
anthranilate | at 87°C | Saccharolobus solfataricus | |
0.4 | - |
5-phospho-alpha-D-ribose 1-diphosphate | 87°C | Saccharolobus solfataricus | |
0.4 | - |
anthranilate | 87°C | Saccharolobus solfataricus |