Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Arabidopsis thaliana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,2,4-Triazole-3-alanine | - |
Arabidopsis thaliana | |
L-histidine | feed-back inhibition; feed-back inhibition | Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.13 | - |
5-phospho-alpha-D-ribose 1-diphosphate | recombinant At-ATP-PRT1 | Arabidopsis thaliana | |
0.51 | - |
ATP | crude cell extracts | Arabidopsis thaliana | |
0.57 | - |
5-phospho-alpha-D-ribose 1-diphosphate | crude cell extracts | Arabidopsis thaliana | |
0.6 | - |
ATP | recombinant enzyme | Arabidopsis thaliana | |
37 | - |
5-phospho-alpha-D-ribose 1-diphosphate | crude cell extracts | Arabidopsis thaliana | |
89 | - |
ATP | crude cell extracts | Arabidopsis thaliana |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
44600 | - |
calculated mass from amino acid sequence | Arabidopsis thaliana |
44800 | - |
calculated mass from amino acid sequence | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 5-phospho-alpha-D-ribose 1-diphosphate | Arabidopsis thaliana | first step in histidine biosynthesis | diphosphate + N-1-(5'-phosphoribosyl)-ATP | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
ecotype Columbia | - |
Arabidopsis thaliana | - |
At-ATP-PRT2 | - |
Arabidopsis thaliana | Q9S762 | ecotype Columbia | - |
Arabidopsis thaliana | Q9S762 | At-ATP-PRT1 | - |
Purification (Comment) | Organism |
---|---|
the recombinant enzymes are produced as fused proteins with a maltose-binding protein, and the purification is made by a two step amylose resin column followed by a digestion with Factor Xa | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Arabidopsis thaliana | 1-(5-phospho-D-ribosyl)-ATP + diphosphate | - |
? | |
ATP + 5-phospho-alpha-D-ribose 1-diphosphate | first step in histidine biosynthesis | Arabidopsis thaliana | diphosphate + N-1-(5'-phosphoribosyl)-ATP | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
assay at | Arabidopsis thaliana |