Literature summary for 2.4.2.14 extracted from

Cao, X.; Du, B.; Han, F.; Zhou, Y.; Ren, J.; Wang, W.; Chen, Z.; Zhang, Y.
Crystal structure of the chloroplastic glutamine phosphoribosylpyrophosphate amidotransferase GPRAT2 from Arabidopsis thaliana (2020), Front. Plant Sci., 11, 157 .

Search for more literature for 2.4.2.14

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21 cells	Arabidopsis thaliana

Crystallization (Commentary)

Crystallization (Comment)	Organism
sitting drop vapor diffusion method, using 0.1 M sodium citrate tribasic pH 5.6, 1.5 M ammonium phosphate monobasic, and 0.1 M citric acid pH 3.4	Arabidopsis thaliana

Protein Variants

Protein Variants	Comment	Organism
C87A	the mutant shows strongly reduced activity compared to the wild type enzyme	Arabidopsis thaliana
D432A	the mutant shows strongly reduced activity compared to the wild type enzyme	Arabidopsis thaliana
D433A	the mutant shows strongly reduced activity compared to the wild type enzyme	Arabidopsis thaliana
S434A	the mutant shows strongly reduced activity compared to the wild type enzyme	Arabidopsis thaliana
Y329A	the mutant shows strongly reduced activity compared to the wild type enzyme	Arabidopsis thaliana

Inhibitors

Inhibitors	Comment	Organism	Structure
6-diazo-5-oxo-L-norleucine	-	Arabidopsis thaliana
acivicin	-	Arabidopsis thaliana
azaserine	-	Arabidopsis thaliana
DAS734	i.e. [5-(4-chlorophenyl)-1-isopropyl-1H-[1,2,4]triazol-3-yl]-acetic acid, direct and specific inhibition, competitive inhibitor for the substrate 5-phospho-alpha-D-ribose 1-diphosphate	Arabidopsis thaliana

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.35	-	5-phospho-alpha-D-ribose 1-diphosphate	at pH 7.8, temperature not specified in the publication	Arabidopsis thaliana

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
chloroplast	-	Arabidopsis thaliana	9507	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	5 mM used in assay conditions	Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O	Arabidopsis thaliana	-	5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate	-	?

Organism

Organism	UniProt	Comment	Textmining
Arabidopsis thaliana	Q9STG9	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-affinity column chromatography	Arabidopsis thaliana

Source Tissue

Source Tissue	Comment	Organism	Textmining

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O	-	Arabidopsis thaliana	5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate	-	?

Synonyms

Synonyms	Comment	Organism
glutamine phosphoribosylpyrophosphate amidotransferase	-	Arabidopsis thaliana
GPRAT2	-	Arabidopsis thaliana

Cofactor

Cofactor	Comment	Organism	Structure
[4Fe-4S]-center	-	Arabidopsis thaliana

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.005293	-	DAS734	at pH 7.8, temperature not specified in the publication	Arabidopsis thaliana

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Release 2023.1 (January 2023)