General Stability | Organism |
---|---|
stability profile depends on growth rate and growth phase of cell culture, PMSF improves stability | Schizosaccharomyces pombe |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
AMP | 2.7 mM and 1.2 mM, 50% inhibition of stable and unstable enzyme preparation respectively | Schizosaccharomyces pombe | |
GMP | 0.44 mM and 0.25 mM, 50% inhibition of stable and unstable enzyme preparation respectively | Schizosaccharomyces pombe |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
180000 | - |
mutant unstable enzyme, gel filtration | Schizosaccharomyces pombe |
360000 | - |
wild-type stable enzyme, gel filtration | Schizosaccharomyces pombe |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Schizosaccharomyces pombe | - |
wild type strain 972h- and mutant strain ade 2h- devoid of adenylosuccinate synthase | - |
Purification (Comment) | Organism |
---|---|
streptomycin, ammonium sulfate, Sephadex g-200 | Schizosaccharomyces pombe |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.02 | - |
- |
Schizosaccharomyces pombe |
Storage Stability | Organism |
---|---|
3°C, crude mutant enzyme extract, inactivation overnight, partially purified mutant enzyme: inactivation within 72 h, PMSF stabilizes | Schizosaccharomyces pombe |
3°C, crude or partially purified wild-type enzyme, at least 72 h stable | Schizosaccharomyces pombe |
PMSF stabilizes during storage | Schizosaccharomyces pombe |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O | - |
Schizosaccharomyces pombe | 5-phospho-beta-D-ribosylamine + L-glutamate + diphosphate | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
48 | - |
wild-type, stable enzyme from cells of mid-exponential growth phase, t1/2: 34 min, from cells of stationary growth phase, t1/2: 2-5 min, mutant enzyme, t1/2: 2 min, in the presence of ADP or adenosine 3 min, and 4 min in the presence of IMP | Schizosaccharomyces pombe |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
unstable enzyme | Schizosaccharomyces pombe |
8 | - |
stable enzyme | Schizosaccharomyces pombe |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 9 | stable enzyme, approx. 65% and unstable enzyme approx. 70% of maximal activity at pH 7, stable enzyme, approx. 75% and unstable enzyme approx. 65% of maximal activity at pH 9 | Schizosaccharomyces pombe |