Inhibitors | Comment | Organism | Structure |
---|---|---|---|
6-diazo-5-oxo-L-norleucine | competitive vs. glutamine, inactivation half-life: 11 min | Homo sapiens | |
AMP | 5 mM, 79% and 24% inhibition of aminotransferase and amidotransferase activity at 1 mM 5-phospho-alpha-D-ribose 1-diphosphate | Homo sapiens | |
azaserine | competitive vs. glutamine, inactivation half-life: 12 min | Homo sapiens | |
GMP | 0.5 mM; 5 mM, 56% and 12% inhibition of aminotransferase and amidotransferase activity at 1 mM 5-phospho-alpha-D-ribose 1-diphosphate | Homo sapiens | |
NH3 | inhibition of amidotransferase activity | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetic properties of amido- and aminotransferase activity | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
DEAE-cellulose, partial purification | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
placenta | - |
Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.001 | - |
L-glutamine | Homo sapiens |
0.00297 | - |
NH3 | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O | no activity with carbamoyl phosphate | Homo sapiens | 5-phospho-beta-D-ribosylamine + L-glutamate + diphosphate | - |
? | |
L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O | reaction at 33% the rate of amminotransferase activity | Homo sapiens | 5-phospho-beta-D-ribosylamine + L-glutamate + diphosphate | - |
? | |
NH3 + 5-phospho-alpha-D-ribose 1-diphosphate + H2O | 3fold higher aminotransferase activity compared to amidotransferase activity | Homo sapiens | 5-phospho-beta-D-ribosylamine + diphosphate | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0034 | - |
6-diazo-5-oxo-L-norleucine | - |
Homo sapiens | |
3.3 | - |
NH3 | - |
Homo sapiens | |
4 | - |
azaserine | - |
Homo sapiens |