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Literature summary for 2.4.2.14 extracted from

  • Holmes, E.W.; McDonald, J.A.; McCord, J.M.; Wyngaarden, J.B.; Kelley, W.N.
    Human glutamine phosphoribosylpyrophosphate amidotransferase. Kinetic and regulatory properties (1973), J. Biol. Chem., 248, 144-150.
    View publication on PubMed
Search for more literature for 2.4.2.14

General Stability

General Stability Organism
Mg2+ and phosphate are essential for stability Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
6-iodopurine 5 mM, 39% inhibition Homo sapiens
6-mercaptopurine ribonucleotide 5 mM, 64% inhibition Homo sapiens
ADP 5 mM, 40% inhibition Homo sapiens
allopurinol ribonucleotide 5 mM, 68% inhibition Homo sapiens
AMP 5 mM, 76% inhibition, noncompetive vs. glutamine Homo sapiens
ATP 5 mM, 21% inhibition Homo sapiens
CMP 5 mM, 22% inhibition Homo sapiens
dCMP 5 mM, 26% inhibition Homo sapiens
GDP 5 mM, 40% inhibition Homo sapiens
GMP 0.5 mM; 5 mM, 70% inhibition Homo sapiens
GTP 5 mM, 13% inhibition Homo sapiens
IDP 5 mM, 29% inhibition Homo sapiens
IMP 5 mM, 41% inhibition Homo sapiens
methyl-dCMP 5 mM, 47% inhibition Homo sapiens
OMP 5 mM, 25% inhibition Homo sapiens
phosphate competitive vs. 5-phospho-alpha-D-ribose 1-diphosphate Homo sapiens
TMP 5 mM, 42% inhibition Homo sapiens
TTP 5 mM, 12% inhibition Homo sapiens
UDP 5 mM, 19% inhibition Homo sapiens
UMP 5 mM, 25% inhibition Homo sapiens
UTP 5 mM, 16% inhibition Homo sapiens
XDP 5 mM, 27% inhibition Homo sapiens
XMP 5 mM, 41% inhibition Homo sapiens
XTP 5 mM, 44% inhibition Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information effect of AMP on kinetic parameters Homo sapiens
0.48
-
 5-phospho-alpha-D-ribose 1-diphosphate in Tris buffer Homo sapiens
0.65
-
 Mg2+
-
 Homo sapiens
1 4.5 L-glutamine
-
 Homo sapiens
1.6
-
 L-glutamine
-
 Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol little or no activity in mitochondria or microsomes Homo sapiens 5829
-
soluble
-
 Homo sapiens
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activation, approx. 15% as effective as Mg2+ or Mn2+ Homo sapiens
Co2+ activation, approx. 40% as effective as Mg2+ or Mn2+ Homo sapiens
Mg2+ required Homo sapiens
Mg2+ Km-value: 0.65 mM, inhibition above 10 mM Homo sapiens
Mn2+ required, equally effective as Mg2+ Homo sapiens
additional information not activated by Ba2+, Cd2+, Cu2+, Fe2+, Hg2+, Ni2+, Zn2+ Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O Homo sapiens regulating enzyme of purine biosynthesis L-glutamate + 5-phospho-beta-D-ribosylamine + diphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
placenta
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0055
-
-
 Homo sapiens

Storage Stability

Storage Stability Organism
4°C, 50 mM phosphate bufer, pH 7.4, 5 mM MgCl2, 60 mM 2-mercaptoethanol, at least 4 weeks, no loss of activity Homo sapiens
4°C, partially purified preparation in 50 mM phosphate buffer, pH 7.4, 5 mM Mg2+ and 60 mM 2-mercaptoethanol, at least 4 weeks Homo sapiens
4°C, phosphate buffer, 10 d, 40% loss of activity Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O
-
 Homo sapiens 5-phospho-beta-D-ribosylamine + L-glutamate + diphosphate
-
 ?
L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O regulating enzyme of purine biosynthesis Homo sapiens L-glutamate + 5-phospho-beta-D-ribosylamine + diphosphate
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 8 broad, phosphate buffer Homo sapiens
6.5
-
 imidazole/HCl buffer Homo sapiens
7.5
-
 Tris/HCl buffer Homo sapiens