Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli Rosetta 2 (DE3) | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
DELTA D93 | naturally occuring mutation in CHS-828 or APO866 resistant cell lines, about 20% of wild type aczivity | Homo sapiens |
H191R | activity reduced by about 50% | Homo sapiens |
H191R/K342R | naturally occuring mutation in APO866 resistant cell line | Homo sapiens |
H191R/K342R/Q388R | naturally occuring mutation in TP201565 resistant cell line | Homo sapiens |
K342R | naturally occuring mutation, activity reduced by about 50% | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CHS-828 | competitive inhibitor | Homo sapiens | |
FK-866 | - |
Homo sapiens | |
TP201565 | competitive inhibitor | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P43490 | - |
- |
Purification (Comment) | Organism |
---|---|
immobilized metal ion affinity chromatography (Ni2+) | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
HCT-116 cell | - |
Homo sapiens | - |
HEK-293T cell | - |
Homo sapiens | - |
NYH cell | - |
Homo sapiens | - |
PC-3 cell | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
nicotinamide + 5-phosphoribosyl 1-diphosphate | - |
Homo sapiens | nicotinamide mononucleotide + diphosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
nicotinamide phosphoribosyltransferase | - |
Homo sapiens |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.00011 | - |
wild type protein, pH 8.5, 37°C | Homo sapiens | FK-866 | |
0.008585 | - |
H191R mutant protein, pH 8.5, 37°C | Homo sapiens | FK-866 |