Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Mycobacterium tuberculosis |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method. Enzyme in complex with phosphate: 0.1 M HEPES pH 7.5, 10% (w/v) PEG8000, 8% (v/v) ethylene glycol. Enzyme in complex with 5-phospho-alpha-D-ribose 1-diphosphate: 0.1 M HEPES pH 7.5, 10% (w/v) PEG6000, 5% (v/v) 2-methyl-2,4-pentanediol. Enzyme in complex with ferric dicitrate: 0.01 M iron(III) chloride hexahydrate, 0.1 M sodium citrate tribasic dehydrate pH 6.1, 10% (v/v) and Jeffamine M-600 | Mycobacterium tuberculosis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required for activity, 20 mM used in assay conditions | Mycobacterium tuberculosis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
orotate + 5-phospho-alpha-D-ribose 1-diphosphate | Mycobacterium tuberculosis | - |
orotidine 5'-phosphate + diphosphate | - |
r | |
orotate + 5-phospho-alpha-D-ribose 1-diphosphate | Mycobacterium tuberculosis H37Rv | - |
orotidine 5'-phosphate + diphosphate | - |
r | |
orotidine 5'-phosphate + diphosphate | Mycobacterium tuberculosis | - |
orotate + 5-phospho-alpha-D-ribose 1-diphosphate | - |
r | |
orotidine 5'-phosphate + diphosphate | Mycobacterium tuberculosis H37Rv | - |
orotate + 5-phospho-alpha-D-ribose 1-diphosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | P9WHK9 | - |
- |
Mycobacterium tuberculosis H37Rv | P9WHK9 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography and Sephacryl 200 gel filtration | Mycobacterium tuberculosis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2 | - |
crude enzyme, at pH 8.0, temperature not specified in the publication | Mycobacterium tuberculosis |
17 | - |
after 8.5fold purification, at pH 8.0, temperature not specified in the publication | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
orotate + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Mycobacterium tuberculosis | orotidine 5'-phosphate + diphosphate | - |
r | |
orotate + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Mycobacterium tuberculosis H37Rv | orotidine 5'-phosphate + diphosphate | - |
r | |
orotidine 5'-phosphate + diphosphate | - |
Mycobacterium tuberculosis | orotate + 5-phospho-alpha-D-ribose 1-diphosphate | - |
r | |
orotidine 5'-phosphate + diphosphate | - |
Mycobacterium tuberculosis H37Rv | orotate + 5-phospho-alpha-D-ribose 1-diphosphate | - |
r |
Synonyms | Comment | Organism |
---|---|---|
OPRT | - |
Mycobacterium tuberculosis |
PyrE | - |
Mycobacterium tuberculosis |
rv0382c | - |
Mycobacterium tuberculosis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
52 | - |
melting temperature | Mycobacterium tuberculosis |