Cloned (Comment) | Organism |
---|---|
OPRT, stable overexpression in the gastric cancer cell lines TMK-1 and MKN-45, enhancing the effect of 5-fluorouracil on the cells, transfected cells show a similar growth curve as the wild-type parent cells, while the drug sensitivity is increased compared to the untransfected cells, overview | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
52000 | - |
x * 52000, SDS-PAGE | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-fluorouracil + 5-phospho-alpha-D-ribose 1-diphosphate | Homo sapiens | - |
5-fluorouridine 5'-phosphate + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
gastric cancer cell | - |
Homo sapiens | - |
MKN-45 cell | gastric cancer cell line with low baseline expression levels of OPRT | Homo sapiens | - |
TMK-1 cell | gastric cancer cell line with low baseline expression levels of OPRT | Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0000045 | - |
untransfected TMK-1 cells | Homo sapiens |
0.000009 | - |
untransfected MKN-45 cells | Homo sapiens |
0.000072 | - |
transfected MKN-45-OPRT cells | Homo sapiens |
0.000172 | - |
transfected TMK-OPRT cells | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-fluorouracil + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Homo sapiens | 5-fluorouridine 5'-phosphate + diphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 52000, SDS-PAGE | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
OPRT | - |
Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Homo sapiens |