Cloned (Comment) | Organism |
---|---|
- |
Plasmodium falciparum |
General Stability | Organism |
---|---|
native enzyme complex, stable for at least 6 months at -20°C or -80°C in 50 mM Hepes, pH 7.4, 300 mM NaCl, 5 mM dithiothreitol, 20% glycerol. At 4°C, loss of 50% of activity within 4 weeks. | Plasmodium falciparum |
recombinant enzyme dimer, stable for at least 3 months at -20°C or -80°C in 50 mM Hepes, pH 7.4, 5 mM dithiothreitol, 20% glycerol. In absence of dithiothreitol and glycerol, loss of 50% of activity during overnight storage at 4°C. | Plasmodium falciparum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0045 | - |
Orotate | 37 °C, pH 8.0, mixture of recombinant enzyme subunits | Plasmodium falciparum | |
0.0056 | - |
Orotate | 37 °C, pH 8.0, native enzyme complex | Plasmodium falciparum | |
0.0113 | - |
5-phospho-alpha-D-ribose 1-diphosphate | 37 °C, pH 8.0, native enzyme complex | Plasmodium falciparum | |
0.0164 | - |
5-phospho-alpha-D-ribose 1-diphosphate | 37 °C, pH 8.0, mixture of recombinant enzyme subunits | Plasmodium falciparum | |
0.0182 | - |
Orotate | 37 °C, pH 8.0, recombinant monofunctional enzyme | Plasmodium falciparum | |
0.0286 | - |
5-phospho-alpha-D-ribose 1-diphosphate | 37 °C, pH 8.0, recombinant monofunctional enzyme | Plasmodium falciparum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
32000 | - |
2 * 32000, enzyme subunit, + 2 * 38000, orotidine 5-monophosphate decarboxylase subunit, SDS-PAGE, native enzyme | Plasmodium falciparum |
32000 | - |
2 * 32000, SDS-PAGE, recombinant enzyme | Plasmodium falciparum |
38000 | - |
2 * 32000, enzyme subunit, + 2 * 38000, orotidine 5-monophosphate decarboxylase subunit, SDS-PAGE, native enzyme | Plasmodium falciparum |
67000 | - |
gel filtration, recombinant enzyme | Plasmodium falciparum |
140000 | - |
gel filtration and SDS-PAGE of native enzyme crosslinked with dimethyl suberimidate | Plasmodium falciparum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Plasmodium falciparum | Q8T6J6 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
orotate + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Plasmodium falciparum | orotidine 5'-phosphate + diphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 32000, SDS-PAGE, recombinant enzyme | Plasmodium falciparum |
tetramer | 2 * 32000, enzyme subunit, + 2 * 38000, orotidine 5-monophosphate decarboxylase subunit, SDS-PAGE, native enzyme | Plasmodium falciparum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.2 | - |
Orotate | 37 °C, pH 8.0, recombinant monofunctional enzyme | Plasmodium falciparum | |
6 | - |
Orotate | 37 °C, pH 8.0, mixture of recombinant enzyme subunits | Plasmodium falciparum | |
10.8 | - |
Orotate | 37 °C, pH 8.0, native enzyme complex | Plasmodium falciparum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 8 | recombinant monofunctional enzyme | Plasmodium falciparum |