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Literature summary for 2.4.2.10 extracted from

  • Krungkrai, S.R.; DelFraino, B.J.; Smiley, J.A.; Prapunwattana, P.; Mitamura, T.; Horii, T.; Krungkrai, J.
    A novel enzyme complex of orotate phosphoribosyltransferase and orotidine 5'-monophosphate decarboxylase in human malaria parasite Plasmodium falciparum: physical association, kinetics, and inhibition characterization (2005), Biochemistry, 44, 1643-1652.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
-
Plasmodium falciparum

General Stability

General Stability Organism
native enzyme complex, stable for at least 6 months at -20°C or -80°C in 50 mM Hepes, pH 7.4, 300 mM NaCl, 5 mM dithiothreitol, 20% glycerol. At 4°C, loss of 50% of activity within 4 weeks. Plasmodium falciparum
recombinant enzyme dimer, stable for at least 3 months at -20°C or -80°C in 50 mM Hepes, pH 7.4, 5 mM dithiothreitol, 20% glycerol. In absence of dithiothreitol and glycerol, loss of 50% of activity during overnight storage at 4°C. Plasmodium falciparum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0045
-
Orotate 37 °C, pH 8.0, mixture of recombinant enzyme subunits Plasmodium falciparum
0.0056
-
Orotate 37 °C, pH 8.0, native enzyme complex Plasmodium falciparum
0.0113
-
5-phospho-alpha-D-ribose 1-diphosphate 37 °C, pH 8.0, native enzyme complex Plasmodium falciparum
0.0164
-
5-phospho-alpha-D-ribose 1-diphosphate 37 °C, pH 8.0, mixture of recombinant enzyme subunits Plasmodium falciparum
0.0182
-
Orotate 37 °C, pH 8.0, recombinant monofunctional enzyme Plasmodium falciparum
0.0286
-
5-phospho-alpha-D-ribose 1-diphosphate 37 °C, pH 8.0, recombinant monofunctional enzyme Plasmodium falciparum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32000
-
2 * 32000, enzyme subunit, + 2 * 38000, orotidine 5’-monophosphate decarboxylase subunit, SDS-PAGE, native enzyme Plasmodium falciparum
32000
-
2 * 32000, SDS-PAGE, recombinant enzyme Plasmodium falciparum
38000
-
2 * 32000, enzyme subunit, + 2 * 38000, orotidine 5’-monophosphate decarboxylase subunit, SDS-PAGE, native enzyme Plasmodium falciparum
67000
-
gel filtration, recombinant enzyme Plasmodium falciparum
140000
-
gel filtration and SDS-PAGE of native enzyme crosslinked with dimethyl suberimidate Plasmodium falciparum

Organism

Organism UniProt Comment Textmining
Plasmodium falciparum Q8T6J6
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
orotate + 5-phospho-alpha-D-ribose 1-diphosphate
-
Plasmodium falciparum orotidine 5'-phosphate + diphosphate
-
?

Subunits

Subunits Comment Organism
dimer 2 * 32000, SDS-PAGE, recombinant enzyme Plasmodium falciparum
tetramer 2 * 32000, enzyme subunit, + 2 * 38000, orotidine 5’-monophosphate decarboxylase subunit, SDS-PAGE, native enzyme Plasmodium falciparum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.2
-
Orotate 37 °C, pH 8.0, recombinant monofunctional enzyme Plasmodium falciparum
6
-
Orotate 37 °C, pH 8.0, mixture of recombinant enzyme subunits Plasmodium falciparum
10.8
-
Orotate 37 °C, pH 8.0, native enzyme complex Plasmodium falciparum

pH Range

pH Minimum pH Maximum Comment Organism
6 8 recombinant monofunctional enzyme Plasmodium falciparum