Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21-Codon plus cells | Plasmodium falciparum |
expressed in Escherichia coli BL21-Codon plus cells | Toxoplasma gondii |
Crystallization (Comment) | Organism |
---|---|
in complex with phosphate and immucillin-H, sitting drop vapor diffusion method, using 25% (w/v) polyethylene glycol monomethyl ether 2000, 100 mM Tris (pH 8.5), and 0.2 M trimethylamine N-oxide dihydrate | Plasmodium falciparum |
in complex with phosphate and immucillin-H, sitting drop vapor diffusion method, using 25% (w/v) polyethylene glycol monomethyl ether 2000, 100 mM Tris (pH 8.5), and 0.2 M trimethylamine N-oxide dihydrate | Toxoplasma gondii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1',9-methyl-immucillin-H | - |
Plasmodium falciparum | |
1',9-methyl-immucillin-H | - |
Toxoplasma gondii | |
2'-deoxy-immucillin-H | - |
Plasmodium falciparum | |
2'-deoxy-immucillin-H | - |
Toxoplasma gondii | |
4'-deaza-1'-aza-2'-deoxy-1',9-methyl-immucillin-G | - |
Plasmodium falciparum | |
4'-deaza-1'-aza-2'-deoxy-1',9-methyl-immucillin-G | - |
Toxoplasma gondii | |
4'-deaza-1'-aza-2'-deoxy-1',9-methyl-immucillin-H | - |
Plasmodium falciparum | |
4'-deaza-1'-aza-2'-deoxy-1',9-methyl-immucillin-H | - |
Toxoplasma gondii | |
5'-amido-immucillin-H | - |
Plasmodium falciparum | |
5'-amido-immucillin-H | - |
Toxoplasma gondii | |
5'-carboxy-immucillin-H | - |
Plasmodium falciparum | |
5'-carboxy-immucillin-H | - |
Toxoplasma gondii | |
5'-deoxy-immucillin-H | - |
Plasmodium falciparum | |
5'-deoxy-immucillin-H | - |
Toxoplasma gondii | |
5'-fluoro-immucillin-H | - |
Plasmodium falciparum | |
5'-fluoro-immucillin-H | - |
Toxoplasma gondii | |
5'-methylthio-immucillin-H | - |
Plasmodium falciparum | |
5'-methylthio-immucillin-H | - |
Toxoplasma gondii | |
5'-thio-immucillin-H | - |
Plasmodium falciparum | |
5'-thio-immucillin-H | - |
Toxoplasma gondii | |
immucillin-H | transition-state analogue inhibitor | Plasmodium falciparum | |
immucillin-H | transition-state analogue inhibitor | Toxoplasma gondii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.004 | - |
Inosine | pH 7.4, temperature not specified in the publication | Toxoplasma gondii | |
0.011 | - |
Inosine | pH 7.4, temperature not specified in the publication | Plasmodium falciparum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Plasmodium falciparum | - |
- |
- |
Toxoplasma gondii | Q2HXR2 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA affinity column chromatography, and gel filtration | Plasmodium falciparum |
Ni-NTA affinity column chromatography, and gel filtration | Toxoplasma gondii |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
tachyzoite | - |
Plasmodium falciparum | - |
tachyzoite | - |
Toxoplasma gondii | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
inosine + phosphate | - |
Plasmodium falciparum | hypoxanthine + alpha-D-ribose 1-phosphate | - |
r | |
inosine + phosphate | - |
Toxoplasma gondii | hypoxanthine + alpha-D-ribose 1-phosphate | - |
r |
Synonyms | Comment | Organism |
---|---|---|
PNP | - |
Plasmodium falciparum |
PNP | - |
Toxoplasma gondii |
purine nucleoside phosphorylase | - |
Plasmodium falciparum |
purine nucleoside phosphorylase | - |
Toxoplasma gondii |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.23 | - |
Inosine | pH 7.4, temperature not specified in the publication | Toxoplasma gondii | |
1.7 | - |
Inosine | pH 7.4, temperature not specified in the publication | Plasmodium falciparum |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000000093 | - |
5'-thio-immucillin-H | pH 7.4, temperature not specified in the publication | Plasmodium falciparum | |
0.00000027 | - |
5'-methylthio-immucillin-H | pH 7.4, temperature not specified in the publication | Plasmodium falciparum | |
0.00000037 | - |
immucillin-H | pH 7.4, temperature not specified in the publication | Toxoplasma gondii | |
0.0000005 | - |
4'-deaza-1'-aza-2'-deoxy-1',9-methyl-immucillin-H | pH 7.4, temperature not specified in the publication | Plasmodium falciparum | |
0.00000086 | - |
immucillin-H | pH 7.4, temperature not specified in the publication | Plasmodium falciparum | |
0.0000009 | - |
4'-deaza-1'-aza-2'-deoxy-1',9-methyl-immucillin-G | pH 7.4, temperature not specified in the publication | Plasmodium falciparum | |
0.0000011 | - |
5'-deoxy-immucillin-H | pH 7.4, temperature not specified in the publication | Toxoplasma gondii | |
0.0000016 | - |
5'-deoxy-immucillin-H | pH 7.4, temperature not specified in the publication | Plasmodium falciparum | |
0.0000019 | - |
2'-deoxy-immucillin-H | pH 7.4, temperature not specified in the publication | Plasmodium falciparum | |
0.000002 | - |
5'-fluoro-immucillin-H | pH 7.4, temperature not specified in the publication | Toxoplasma gondii | |
0.0000022 | - |
5'-fluoro-immucillin-H | pH 7.4, temperature not specified in the publication | Plasmodium falciparum | |
0.000012 | - |
5'-carboxy-immucillin-H | pH 7.4, temperature not specified in the publication | Toxoplasma gondii | |
0.000017 | - |
5'-carboxy-immucillin-H | pH 7.4, temperature not specified in the publication | Plasmodium falciparum | |
0.000019 | - |
2'-deoxy-immucillin-H | pH 7.4, temperature not specified in the publication | Toxoplasma gondii | |
0.00022 | - |
5'-amido-immucillin-H | pH 7.4, temperature not specified in the publication | Plasmodium falciparum | |
0.0015 | - |
4'-deaza-1'-aza-2'-deoxy-1',9-methyl-immucillin-G | pH 7.4, temperature not specified in the publication | Toxoplasma gondii | |
0.0036 | - |
4'-deaza-1'-aza-2'-deoxy-1',9-methyl-immucillin-H | pH 7.4, temperature not specified in the publication | Toxoplasma gondii | |
0.0056 | - |
5'-methylthio-immucillin-H | pH 7.4, temperature not specified in the publication | Toxoplasma gondii | |
0.0089 | - |
5'-amido-immucillin-H | pH 7.4, temperature not specified in the publication | Toxoplasma gondii | |
0.05 | - |
5'-thio-immucillin-H | Ki above 0.05 mM, pH 7.4, temperature not specified in the publication | Toxoplasma gondii | |
0.05 | - |
1',9-methyl-immucillin-H | Ki above 0.05 mM, pH 7.4, temperature not specified in the publication | Toxoplasma gondii | |
0.6 | - |
1',9-methyl-immucillin-H | Ki above 0.6 mM, pH 7.4, temperature not specified in the publication | Plasmodium falciparum |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
15 | - |
Inosine | pH 7.4, temperature not specified in the publication | Plasmodium falciparum | |
55 | - |
Inosine | pH 7.4, temperature not specified in the publication | Toxoplasma gondii |