Application | Comment | Organism |
---|---|---|
synthesis | the enzyme is a valuable industrial biocatalysts for high-temperature reactions that produce nucleoside drugs in high yields | Aeropyrum pernix |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Aeropyrum pernix |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.318 | - |
Inosine | pH 7.0, 90°C | Aeropyrum pernix | |
0.578 | - |
adenosine | pH 7.0, 90°C | Aeropyrum pernix | |
0.69 | - |
guanosine | pH 7.0, 90°C | Aeropyrum pernix |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30000 | - |
gel filtration | Aeropyrum pernix |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aeropyrum pernix | Q9YDC0 | - |
- |
Aeropyrum pernix DSM 11879 | Q9YDC0 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Aeropyrum pernix |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2'-deoxyadenosine + phosphate | - |
Aeropyrum pernix | adenine + 2-deoxy-alpha-D-ribose 1-phosphate | - |
? | |
2'-deoxyadenosine + phosphate | - |
Aeropyrum pernix DSM 11879 | adenine + 2-deoxy-alpha-D-ribose 1-phosphate | - |
? | |
2,6-diaminopurine nucleoside + phosphate | - |
Aeropyrum pernix | alpha-D-ribose 1-phosphate + 2,6-diaminopurine | - |
? | |
adenosine + phosphate | - |
Aeropyrum pernix | adenine + alpha-D-ribose 1-phosphate | - |
? | |
adenosine + phosphate | - |
Aeropyrum pernix DSM 11879 | adenine + alpha-D-ribose 1-phosphate | - |
? | |
guanosine + phosphate | - |
Aeropyrum pernix | guanine + alpha-D-ribose 1-phosphate | - |
? | |
guanosine + phosphate | - |
Aeropyrum pernix DSM 11879 | guanine + alpha-D-ribose 1-phosphate | - |
? | |
inosine + phosphate | - |
Aeropyrum pernix | hypoxanthine + alpha-D-ribose 1-phosphate | - |
? | |
inosine + phosphate | - |
Aeropyrum pernix DSM 11879 | hypoxanthine + alpha-D-ribose 1-phosphate | - |
? | |
additional information | the enzyme cannot accept 6-chloropurines | Aeropyrum pernix | ? | - |
? | |
additional information | the enzyme cannot accept 6-chloropurines | Aeropyrum pernix DSM 11879 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 30000, SDS-PAGE | Aeropyrum pernix |
Synonyms | Comment | Organism |
---|---|---|
apPNP | - |
Aeropyrum pernix |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
100 | - |
- |
Aeropyrum pernix |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
100 | - |
the recombinant protein has nearly 100% activity when incubated at 100°C for 60 min | Aeropyrum pernix |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
838 | - |
Inosine | pH 7.0, 90°C | Aeropyrum pernix | |
876 | - |
guanosine | pH 7.0, 90°C | Aeropyrum pernix | |
3450 | - |
adenosine | pH 7.0, 90°C | Aeropyrum pernix |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
- |
Aeropyrum pernix |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3 | 11 | the enzyme retains nearly 100% activity between pH 3.0 and 11.0 when incubated at 90 °C for 60 min | Aeropyrum pernix |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1270 | - |
guanosine | pH 7.0, 90°C | Aeropyrum pernix | |
2630 | - |
Inosine | pH 7.0, 90°C | Aeropyrum pernix | |
5997 | - |
adenosine | pH 7.0, 90°C | Aeropyrum pernix |