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Literature summary for 2.4.2.1 extracted from

  • Zhu, S.; Song, D.; Gong, C.; Tang, P.; Li, X.; Wang, J.; Zheng, G.
    Biosynthesis of nucleoside analogues via thermostable nucleoside phosphorylase (2013), Appl. Microbiol. Biotechnol., 97, 6769-6778.
    View publication on PubMed

Application

Application Comment Organism
synthesis the enzyme is a valuable industrial biocatalysts for high-temperature reactions that produce nucleoside drugs in high yields Aeropyrum pernix

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Aeropyrum pernix

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.318
-
Inosine pH 7.0, 90°C Aeropyrum pernix
0.578
-
adenosine pH 7.0, 90°C Aeropyrum pernix
0.69
-
guanosine pH 7.0, 90°C Aeropyrum pernix

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30000
-
gel filtration Aeropyrum pernix

Organism

Organism UniProt Comment Textmining
Aeropyrum pernix Q9YDC0
-
-
Aeropyrum pernix DSM 11879 Q9YDC0
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Aeropyrum pernix

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2'-deoxyadenosine + phosphate
-
Aeropyrum pernix adenine + 2-deoxy-alpha-D-ribose 1-phosphate
-
?
2'-deoxyadenosine + phosphate
-
Aeropyrum pernix DSM 11879 adenine + 2-deoxy-alpha-D-ribose 1-phosphate
-
?
2,6-diaminopurine nucleoside + phosphate
-
Aeropyrum pernix alpha-D-ribose 1-phosphate + 2,6-diaminopurine
-
?
adenosine + phosphate
-
Aeropyrum pernix adenine + alpha-D-ribose 1-phosphate
-
?
adenosine + phosphate
-
Aeropyrum pernix DSM 11879 adenine + alpha-D-ribose 1-phosphate
-
?
guanosine + phosphate
-
Aeropyrum pernix guanine + alpha-D-ribose 1-phosphate
-
?
guanosine + phosphate
-
Aeropyrum pernix DSM 11879 guanine + alpha-D-ribose 1-phosphate
-
?
inosine + phosphate
-
Aeropyrum pernix hypoxanthine + alpha-D-ribose 1-phosphate
-
?
inosine + phosphate
-
Aeropyrum pernix DSM 11879 hypoxanthine + alpha-D-ribose 1-phosphate
-
?
additional information the enzyme cannot accept 6-chloropurines Aeropyrum pernix ?
-
?
additional information the enzyme cannot accept 6-chloropurines Aeropyrum pernix DSM 11879 ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 30000, SDS-PAGE Aeropyrum pernix

Synonyms

Synonyms Comment Organism
apPNP
-
Aeropyrum pernix

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
100
-
-
Aeropyrum pernix

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
100
-
the recombinant protein has nearly 100% activity when incubated at 100°C for 60 min Aeropyrum pernix

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
838
-
Inosine pH 7.0, 90°C Aeropyrum pernix
876
-
guanosine pH 7.0, 90°C Aeropyrum pernix
3450
-
adenosine pH 7.0, 90°C Aeropyrum pernix

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
Aeropyrum pernix

pH Range

pH Minimum pH Maximum Comment Organism
3 11 the enzyme retains nearly 100% activity between pH 3.0 and 11.0 when incubated at 90 °C for 60 min Aeropyrum pernix

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1270
-
guanosine pH 7.0, 90°C Aeropyrum pernix
2630
-
Inosine pH 7.0, 90°C Aeropyrum pernix
5997
-
adenosine pH 7.0, 90°C Aeropyrum pernix