Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
Y88A | mutation introduced to render enzyme susceptible to substrate 2',3'-dideoxyinosine | Homo sapiens |
Y88C | mutation introduced to render enzyme susceptible to substrate 2',3'-dideoxyinosine | Homo sapiens |
Y88D | mutation introduced to render enzyme susceptible to substrate 2',3'-dideoxyinosine | Homo sapiens |
Y88E | mutation introduced to render enzyme susceptible to substrate 2',3'-dideoxyinosine | Homo sapiens |
Y88F | engineering of human enzyme to accept 2',3'-dideoxyinosine as substrate. Mutant displays 9-fold improvement in kcat and greater than 2-fold reduction in KM of 2',3'-dideoxyinosine | Homo sapiens |
Y88H | mutation introduced to render enzyme susceptible to substrate 2',3'-dideoxyinosine | Homo sapiens |
Y88I | mutation introduced to render enzyme susceptible to substrate 2',3'-dideoxyinosine | Homo sapiens |
Y88K | mutation introduced to render enzyme susceptible to substrate 2',3'-dideoxyinosine | Homo sapiens |
Y88L | mutation introduced to render enzyme susceptible to substrate 2',3'-dideoxyinosine | Homo sapiens |
Y88M | mutation introduced to render enzyme susceptible to substrate 2',3'-dideoxyinosine | Homo sapiens |
Y88N | mutation introduced to render enzyme susceptible to substrate 2',3'-dideoxyinosine | Homo sapiens |
Y88Q | mutation introduced to render enzyme susceptible to substrate 2',3'-dideoxyinosine | Homo sapiens |
Y88R | mutation introduced to render enzyme susceptible to substrate 2',3'-dideoxyinosine | Homo sapiens |
Y88S | mutation introduced to render enzyme susceptible to substrate 2',3'-dideoxyinosine | Homo sapiens |
Y88T | mutation introduced to render enzyme susceptible to substrate 2',3'-dideoxyinosine | Homo sapiens |
Y88V | mutation introduced to render enzyme susceptible to substrate 2',3'-dideoxyinosine | Homo sapiens |
Y88W | mutation introduced to render enzyme susceptible to substrate 2',3'-dideoxyinosine | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.048 | - |
Inosine | wild-type, pH 7.0, 25°C | Homo sapiens | |
0.073 | - |
Inosine | mutant Y88F, pH 7.0, 25°C | Homo sapiens | |
0.074 | - |
Inosine | mutant Y88H, pH 7.0, 25°C | Homo sapiens | |
0.08 | - |
Inosine | mutant Y88A, pH 7.0, 25°C | Homo sapiens | |
0.094 | - |
Inosine | mutant Y88L, pH 7.0, 25°C | Homo sapiens | |
0.17 | - |
Inosine | mutant Y88C, pH 7.0, 25°C | Homo sapiens | |
0.345 | - |
Inosine | mutant Y88M, pH 7.0, 25°C | Homo sapiens | |
0.37 | - |
Inosine | mutant Y88R, pH 7.0, 25°C | Homo sapiens | |
0.391 | - |
2',3'-dideoxyinosine | mutant Y88L, pH 7.0, 25°C | Homo sapiens | |
0.43 | - |
Inosine | mutant Y88S, pH 7.0, 25°C | Homo sapiens | |
0.45 | - |
2',3'-dideoxyinosine | mutant Y88F, pH 7.0, 25°C | Homo sapiens | |
0.5 | - |
Inosine | mutant Y88W, pH 7.0, 25°C | Homo sapiens | |
0.645 | - |
2',3'-dideoxyinosine | mutant Y88M, pH 7.0, 25°C | Homo sapiens | |
0.675 | - |
2',3'-dideoxyinosine | mutant Y88H, pH 7.0, 25°C | Homo sapiens | |
0.81 | - |
2',3'-dideoxyinosine | mutant Y88C, pH 7.0, 25°C | Homo sapiens | |
0.845 | - |
Inosine | mutant Y88M, pH 7.0, 25°C | Homo sapiens | |
0.855 | - |
Inosine | mutant Y88Q, pH 7.0, 25°C | Homo sapiens | |
0.86 | - |
2',3'-dideoxyinosine | mutant Y88Q, pH 7.0, 25°C | Homo sapiens | |
0.98 | - |
2',3'-dideoxyinosine | mutant Y88W, pH 7.0, 25°C | Homo sapiens | |
1.03 | - |
2',3'-dideoxyinosine | wild-type, pH 7.0, 25°C | Homo sapiens | |
1.2 | - |
Inosine | mutant Y88T, pH 7.0, 25°C | Homo sapiens | |
1.2 | - |
Inosine | mutant Y88V, pH 7.0, 25°C | Homo sapiens | |
1.42 | - |
Inosine | mutant Y88I, pH 7.0, 25°C | Homo sapiens | |
1.67 | - |
2',3'-dideoxyinosine | mutant Y88A, pH 7.0, 25°C | Homo sapiens | |
2.6 | - |
Inosine | mutant Y88D, pH 7.0, 25°C | Homo sapiens | |
4.06 | - |
2',3'-dideoxyinosine | mutant Y88S, pH 7.0, 25°C | Homo sapiens | |
4.41 | - |
2',3'-dideoxyinosine | mutant Y88V, pH 7.0, 25°C | Homo sapiens | |
4.67 | - |
2',3'-dideoxyinosine | mutant Y88I, pH 7.0, 25°C | Homo sapiens | |
4.86 | - |
Inosine | mutant Y88E, pH 7.0, 25°C | Homo sapiens | |
8.16 | - |
2',3'-dideoxyinosine | mutant Y88T, pH 7.0, 25°C | Homo sapiens | |
9.53 | - |
2',3'-dideoxyinosine | mutant Y88M, pH 7.0, 25°C | Homo sapiens | |
12.7 | - |
2',3'-dideoxyinosine | mutant Y88D, pH 7.0, 25°C | Homo sapiens | |
14.9 | - |
2',3'-dideoxyinosine | mutant Y88E, pH 7.0, 25°C | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P00491 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2',3'-dideoxyinosine + phosphate | - |
Homo sapiens | hypoxanthine + 2,3-dideoxy-alpha-D-ribose 1-phosphate | - |
? | |
inosine + phosphate | - |
Homo sapiens | hypoxanthine + alpha-D-ribose 1-phosphate | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
2',3'-dideoxyinosine | mutant Y88E, pH 7.0, 25°C | Homo sapiens | |
0.01 | - |
Inosine | mutant Y88R, pH 7.0, 25°C | Homo sapiens | |
0.02 | - |
2',3'-dideoxyinosine | mutant Y88D, pH 7.0, 25°C | Homo sapiens | |
0.09 | - |
2',3'-dideoxyinosine | mutant Y88W, pH 7.0, 25°C | Homo sapiens | |
0.15 | - |
2',3'-dideoxyinosine | mutant Y88Q, pH 7.0, 25°C | Homo sapiens | |
0.4 | - |
2',3'-dideoxyinosine | mutant Y88T, pH 7.0, 25°C | Homo sapiens | |
0.5 | - |
2',3'-dideoxyinosine | mutant Y88I, pH 7.0, 25°C | Homo sapiens | |
0.6 | - |
2',3'-dideoxyinosine | mutant Y88V, pH 7.0, 25°C | Homo sapiens | |
0.7 | - |
Inosine | mutant Y88E, pH 7.0, 25°C | Homo sapiens | |
0.8 | - |
Inosine | mutant Y88D, pH 7.0, 25°C | Homo sapiens | |
0.9 | - |
2',3'-dideoxyinosine | wild-type, pH 7.0, 25°C | Homo sapiens | |
1.3 | - |
Inosine | mutant Y88I, pH 7.0, 25°C | Homo sapiens | |
1.4 | - |
Inosine | mutant Y88Q, pH 7.0, 25°C | Homo sapiens | |
1.5 | - |
2',3'-dideoxyinosine | mutant Y88M, pH 7.0, 25°C | Homo sapiens | |
2.3 | - |
Inosine | mutant Y88T, pH 7.0, 25°C | Homo sapiens | |
2.4 | - |
Inosine | mutant Y88V, pH 7.0, 25°C | Homo sapiens | |
2.5 | - |
2',3'-dideoxyinosine | mutant Y88C, pH 7.0, 25°C | Homo sapiens | |
2.5 | - |
2',3'-dideoxyinosine | mutant Y88H, pH 7.0, 25°C | Homo sapiens | |
2.7 | - |
2',3'-dideoxyinosine | mutant Y88S, pH 7.0, 25°C | Homo sapiens | |
2.8 | - |
2',3'-dideoxyinosine | mutant Y88A, pH 7.0, 25°C | Homo sapiens | |
5.2 | - |
2',3'-dideoxyinosine | mutant Y88M, pH 7.0, 25°C | Homo sapiens | |
5.5 | - |
Inosine | mutant Y88C, pH 7.0, 25°C | Homo sapiens | |
5.6 | - |
Inosine | mutant Y88W, pH 7.0, 25°C | Homo sapiens | |
6.1 | - |
2',3'-dideoxyinosine | mutant Y88L, pH 7.0, 25°C | Homo sapiens | |
8.6 | - |
Inosine | mutant Y88H, pH 7.0, 25°C | Homo sapiens | |
8.8 | - |
Inosine | mutant Y88S, pH 7.0, 25°C | Homo sapiens | |
9.7 | - |
Inosine | mutant Y88M, pH 7.0, 25°C | Homo sapiens | |
10.5 | - |
2',3'-dideoxyinosine | mutant Y88F, pH 7.0, 25°C | Homo sapiens | |
12.8 | - |
Inosine | mutant Y88M, pH 7.0, 25°C | Homo sapiens | |
15.9 | - |
Inosine | mutant Y88L, pH 7.0, 25°C | Homo sapiens | |
17.6 | - |
Inosine | mutant Y88A, pH 7.0, 25°C | Homo sapiens | |
28.3 | - |
Inosine | mutant Y88F, pH 7.0, 25°C | Homo sapiens | |
43.9 | - |
Inosine | wild-type, pH 7.0, 25°C | Homo sapiens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00014 | - |
Inosine | mutant Y88E, pH 7.0, 25°C | Homo sapiens | |
0.00029 | - |
Inosine | mutant Y88D, pH 7.0, 25°C | Homo sapiens | |
0.0009 | - |
2',3'-dideoxyinosine | mutant Y88E, pH 7.0, 25°C | Homo sapiens | |
0.0009 | - |
Inosine | mutant Y88I, pH 7.0, 25°C | Homo sapiens | |
0.0017 | - |
Inosine | mutant Y88Q, pH 7.0, 25°C | Homo sapiens | |
0.0018 | - |
2',3'-dideoxyinosine | mutant Y88D, pH 7.0, 25°C | Homo sapiens | |
0.0019 | - |
Inosine | mutant Y88T, pH 7.0, 25°C | Homo sapiens | |
0.0019 | - |
Inosine | mutant Y88V, pH 7.0, 25°C | Homo sapiens | |
0.0114 | - |
Inosine | mutant Y88M, pH 7.0, 25°C | Homo sapiens | |
0.013 | - |
Inosine | mutant Y88W, pH 7.0, 25°C | Homo sapiens | |
0.0203 | - |
Inosine | mutant Y88S, pH 7.0, 25°C | Homo sapiens | |
0.0321 | - |
Inosine | mutant Y88C, pH 7.0, 25°C | Homo sapiens | |
0.0371 | - |
Inosine | mutant Y88M, pH 7.0, 25°C | Homo sapiens | |
0.044 | - |
2',3'-dideoxyinosine | mutant Y88T, pH 7.0, 25°C | Homo sapiens | |
0.091 | - |
2',3'-dideoxyinosine | mutant Y88W, pH 7.0, 25°C | Homo sapiens | |
0.115 | - |
2',3'-dideoxyinosine | mutant Y88I, pH 7.0, 25°C | Homo sapiens | |
0.116 | - |
Inosine | mutant Y88H, pH 7.0, 25°C | Homo sapiens | |
0.144 | - |
2',3'-dideoxyinosine | mutant Y88V, pH 7.0, 25°C | Homo sapiens | |
0.153 | - |
2',3'-dideoxyinosine | mutant Y88M, pH 7.0, 25°C | Homo sapiens | |
0.169 | - |
Inosine | mutant Y88L, pH 7.0, 25°C | Homo sapiens | |
0.177 | - |
2',3'-dideoxyinosine | mutant Y88Q, pH 7.0, 25°C | Homo sapiens | |
0.219 | - |
Inosine | mutant Y88A, pH 7.0, 25°C | Homo sapiens | |
0.389 | - |
Inosine | mutant Y88F, pH 7.0, 25°C | Homo sapiens | |
0.652 | - |
2',3'-dideoxyinosine | mutant Y88S, pH 7.0, 25°C | Homo sapiens | |
0.875 | - |
2',3'-dideoxyinosine | wild-type, pH 7.0, 25°C | Homo sapiens | |
0.921 | - |
Inosine | wild-type, pH 7.0, 25°C | Homo sapiens | |
1.67 | - |
2',3'-dideoxyinosine | mutant Y88A, pH 7.0, 25°C | Homo sapiens | |
3.07 | - |
2',3'-dideoxyinosine | mutant Y88C, pH 7.0, 25°C | Homo sapiens | |
3.67 | - |
2',3'-dideoxyinosine | mutant Y88H, pH 7.0, 25°C | Homo sapiens | |
8.09 | - |
2',3'-dideoxyinosine | mutant Y88M, pH 7.0, 25°C | Homo sapiens | |
15.67 | - |
2',3'-dideoxyinosine | mutant Y88L, pH 7.0, 25°C | Homo sapiens | |
23.14 | - |
2',3'-dideoxyinosine | mutant Y88F, pH 7.0, 25°C | Homo sapiens |