Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.4.2.1 extracted from

  • Xie, X.; Huo, W.; Xia, J.; Xu, Q.; Chen, N.
    Structure-activity relationship of a cold-adapted purine nucleoside phosphorylase by site-directed mutagenesis (2012), Enzyme Microb. Technol., 51, 59-65.
    View publication on PubMed
Search for more literature for 2.4.2.1

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pseudoalteromonas sp.

Crystallization (Commentary)

Crystallization (Comment) Organism
construction of three-dimensional model Pseudoalteromonas sp.

Protein Variants

Protein Variants Comment Organism
T156A about 5fold decrease in kcat/Km Pseudoalteromonas sp.
T156S increase in kcat/Km Pseudoalteromonas sp.
T90A dramatic decrase in kcat/Km Pseudoalteromonas sp.
T90A/T156A 500fold reduction in catalytic activity when compared with wild-type Pseudoalteromonas sp.
T90R increase in kcat/Km Pseudoalteromonas sp.
T90R/T156S 1000fold enhancements in kcat/Km for inosine phosphorolysis Pseudoalteromonas sp.
T90S about 5fold decrease in kcat/Km Pseudoalteromonas sp.
V206I kcat/Km similar to wild-type Pseudoalteromonas sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00001
-
 Inosine mutant T90R/T156A, pH 7.6, 37°C Pseudoalteromonas sp.
0.00002
-
 Inosine mutant T90R, pH 7.6, 37°C Pseudoalteromonas sp.
0.00003
-
 Inosine mutant T156S, pH 7.6, 37°C Pseudoalteromonas sp.
0.00004
-
 Inosine mutant V206I, pH 7.6, 37°C Pseudoalteromonas sp.
0.00039
-
 Inosine wild-type, pH 7.6, 37°C Pseudoalteromonas sp.
0.00092
-
 Inosine mutant T90S, pH 7.6, 37°C Pseudoalteromonas sp.
0.00135
-
 Inosine mutant T156A, pH 7.6, 37°C Pseudoalteromonas sp.
0.104
-
 Inosine mutant T90A/T156A, pH 7.6, 37°C Pseudoalteromonas sp.
143.1
-
 Inosine mutant T90A, pH 7.6, 37°C Pseudoalteromonas sp.

Organism

Organism UniProt Comment Textmining
Pseudoalteromonas sp. C8CPR9
-
-
Pseudoalteromonas sp. XM2107 C8CPR9
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
inosine + phosphate
-
 Pseudoalteromonas sp. hypoxanthine + alpha-D-ribose 1-phosphate
-
 ?
inosine + phosphate
-
 Pseudoalteromonas sp. XM2107 hypoxanthine + alpha-D-ribose 1-phosphate
-
 ?
additional information the complete hydrophobic pocket plays an important role in the catalytic function. A potential transition state structure is present in hydrogen bond between the carboxyl groups of Thr90 in the phosphate binding site Pseudoalteromonas sp. ?
-
 ?
additional information the complete hydrophobic pocket plays an important role in the catalytic function. A potential transition state structure is present in hydrogen bond between the carboxyl groups of Thr90 in the phosphate binding site Pseudoalteromonas sp. XM2107 ?
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.9
-
 Inosine mutant T90A/T156A, pH 7.6, 37°C Pseudoalteromonas sp.
1.2
-
 Inosine mutant T90A, pH 7.6, 37°C Pseudoalteromonas sp.
56.4
-
 Inosine mutant T156A, pH 7.6, 37°C Pseudoalteromonas sp.
62.3
-
 Inosine mutant T90S, pH 7.6, 37°C Pseudoalteromonas sp.
82.4
-
 Inosine mutant V206I, pH 7.6, 37°C Pseudoalteromonas sp.
84.1
-
 Inosine wild-type, pH 7.6, 37°C Pseudoalteromonas sp.
98.4
-
 Inosine mutant T156S, pH 7.6, 37°C Pseudoalteromonas sp.
164.4
-
 Inosine mutant T90R, pH 7.6, 37°C Pseudoalteromonas sp.
324.3
-
 Inosine mutant T90R/T156A, pH 7.6, 37°C Pseudoalteromonas sp.

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.082
-
 Inosine mutant T90A/T156A, pH 7.6, 37°C Pseudoalteromonas sp.
0.087
-
 Inosine mutant T90A, pH 7.6, 37°C Pseudoalteromonas sp.
41.8
-
 Inosine mutant T156A, pH 7.6, 37°C Pseudoalteromonas sp.
67.8
-
 Inosine mutant T90S, pH 7.6, 37°C Pseudoalteromonas sp.
189
-
 Inosine mutant V206I, pH 7.6, 37°C Pseudoalteromonas sp.
215
-
 Inosine wild-type, pH 7.6, 37°C Pseudoalteromonas sp.
3540
-
 Inosine mutant T156S, pH 7.6, 37°C Pseudoalteromonas sp.
11500
-
 Inosine mutant T90R, pH 7.6, 37°C Pseudoalteromonas sp.
43500
-
 Inosine mutant T90R/T156A, pH 7.6, 37°C Pseudoalteromonas sp.