Application | Comment | Organism |
---|---|---|
synthesis | use of agar from four different polymer carriers as a suitable matrix for whole recombinant cell entrapment. Although the immobilization process reduces the substrate affinity and catalytic efficiency of recombinant cells, it can significantly enhance the stability and reusability of these cells. The immobilized whole cell biocatalyst is applied to produce ribavirin, as a model nucleoside synthesis reaction showing relative high productivity of rabavirin and quick reaction time | Escherichia coli |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P0ABP8 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2'-deoxyadenosine + phosphate | 54.7% of the activity with 2'-deoxyinosine | Escherichia coli | adenine + 2-deoxy-alpha-D-ribose 1-phosphate | - |
? | |
2'-deoxyguanosine + phosphate | 83.2% of the activity with 2'-deoxyinosine | Escherichia coli | guanine + 2-deoxy-alpha-D-ribose 1-phosphate | - |
? | |
2'-deoxyinosine + phosphate | - |
Escherichia coli | hypoxanthine + 2-deoxy-alpha-D-ribose 1-phosphate | - |
? | |
adenosine + phosphate | 59.9% of the activity with 2'-deoxyinosine | Escherichia coli | adenine + alpha-D-ribose 1-phosphate | - |
? | |
guanosine + phosphate | 58.7% of the activity with 2'-deoxyinosine | Escherichia coli | guanine + alpha-D-ribose 1-phosphate | - |
? | |
inosine + phosphate | 83% of the activity with 2'-deoxyinosine | Escherichia coli | hypoxanthine + alpha-D-ribose 1-phosphate | - |
? | |
additional information | no substrate: xanthosine, uridine, 2'-deoxythymidine, cytidine | Escherichia coli | ? | - |
? |