KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.53 | - |
guanosine | pH 7.0, 80°C | Thermus thermophilus | |
1.01 | - |
2'-deoxyguanosine | pH 7.0, 80°C | Thermus thermophilus | |
2.06 | - |
2'-deoxyinosine | pH 7.0, 80°C | Thermus thermophilus | |
2.08 | - |
Inosine | pH 7.0, 80°C | Thermus thermophilus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
25415 | - |
6 * 25415, calculated | Thermus thermophilus |
156000 | - |
sedimentation equilibrium anlysis | Thermus thermophilus |
Organic Solvent | Comment | Organism |
---|---|---|
2-methyltetrahydrofuran | 50%, 25% residual activity | Thermus thermophilus |
dimethyl sulfoxide | 50%, 25% residual activity | Thermus thermophilus |
dimethylformamide | 50%, 30% residual activity | Thermus thermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermus thermophilus | Q72IR2 | - |
- |
Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 | Q72IR2 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2'-deoxyguanosine + phosphate | - |
Thermus thermophilus | guanine + 2-deoxy-alpha-D-ribose 1-phosphate | 22.5% phosphorolysis of substrate within 30 min | ? | |
2'-deoxyguanosine + phosphate | - |
Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 | guanine + 2-deoxy-alpha-D-ribose 1-phosphate | 22.5% phosphorolysis of substrate within 30 min | ? | |
2'-deoxyinosine + phosphate | - |
Thermus thermophilus | hypoxanthine + 2-deoxy-alpha-D-ribose 1-phosphate | 40.5% phosphorolysis of substrate within 30 min | ? | |
2'-deoxyinosine + phosphate | - |
Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 | hypoxanthine + 2-deoxy-alpha-D-ribose 1-phosphate | 40.5% phosphorolysis of substrate within 30 min | ? | |
guanosine + phosphate | - |
Thermus thermophilus | guanine + alpha-D-ribose 1-phosphate | 8.7% phosphorolysis of substrate within 30 min | ? | |
guanosine + phosphate | - |
Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 | guanine + alpha-D-ribose 1-phosphate | 8.7% phosphorolysis of substrate within 30 min | ? | |
inosine + phosphate | - |
Thermus thermophilus | hypoxanthine + alpha-D-ribose 1-phosphate | 19.1% phosphorolysis of substrate within 30 min | ? | |
inosine + phosphate | - |
Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 | hypoxanthine + alpha-D-ribose 1-phosphate | 19.1% phosphorolysis of substrate within 30 min | ? | |
additional information | no substrate: acyclovir, benzimidazole, 2,6-diaminopurine | Thermus thermophilus | ? | - |
? | |
additional information | no substrate: acyclovir, benzimidazole, 2,6-diaminopurine | Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | 6 * 25415, calculated | Thermus thermophilus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
half-life 132 h | Thermus thermophilus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
173 | - |
Inosine | pH 7.0, 80°C | Thermus thermophilus | |
296 | - |
guanosine | pH 7.0, 80°C | Thermus thermophilus | |
627 | - |
2'-deoxyinosine | pH 7.0, 80°C | Thermus thermophilus | |
830 | - |
2'-deoxyguanosine | pH 7.0, 80°C | Thermus thermophilus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
83.2 | - |
Inosine | pH 7.0, 80°C | Thermus thermophilus | |
304.3 | - |
2'-deoxyinosine | pH 7.0, 80°C | Thermus thermophilus | |
558.8 | - |
guanosine | pH 7.0, 80°C | Thermus thermophilus | |
822.5 | - |
2'-deoxyguanosine | pH 7.0, 80°C | Thermus thermophilus |