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Literature summary for 2.4.2.1 extracted from

  • Almendros, M.; Berenguer, J.; Sinisterra, J.
    Thermus thermophilus nucleoside phosphorylases active in the synthesis of nucleoside analogues (2012), Appl. Environ. Microbiol., 78, 3128-3135.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.53
-
guanosine pH 7.0, 80°C Thermus thermophilus
1.01
-
2'-deoxyguanosine pH 7.0, 80°C Thermus thermophilus
2.06
-
2'-deoxyinosine pH 7.0, 80°C Thermus thermophilus
2.08
-
Inosine pH 7.0, 80°C Thermus thermophilus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
25415
-
6 * 25415, calculated Thermus thermophilus
156000
-
sedimentation equilibrium anlysis Thermus thermophilus

Organic Solvent Stability

Organic Solvent Comment Organism
2-methyltetrahydrofuran 50%, 25% residual activity Thermus thermophilus
dimethyl sulfoxide 50%, 25% residual activity Thermus thermophilus
dimethylformamide 50%, 30% residual activity Thermus thermophilus

Organism

Organism UniProt Comment Textmining
Thermus thermophilus Q72IR2
-
-
Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 Q72IR2
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2'-deoxyguanosine + phosphate
-
Thermus thermophilus guanine + 2-deoxy-alpha-D-ribose 1-phosphate 22.5% phosphorolysis of substrate within 30 min ?
2'-deoxyguanosine + phosphate
-
Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 guanine + 2-deoxy-alpha-D-ribose 1-phosphate 22.5% phosphorolysis of substrate within 30 min ?
2'-deoxyinosine + phosphate
-
Thermus thermophilus hypoxanthine + 2-deoxy-alpha-D-ribose 1-phosphate 40.5% phosphorolysis of substrate within 30 min ?
2'-deoxyinosine + phosphate
-
Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 hypoxanthine + 2-deoxy-alpha-D-ribose 1-phosphate 40.5% phosphorolysis of substrate within 30 min ?
guanosine + phosphate
-
Thermus thermophilus guanine + alpha-D-ribose 1-phosphate 8.7% phosphorolysis of substrate within 30 min ?
guanosine + phosphate
-
Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 guanine + alpha-D-ribose 1-phosphate 8.7% phosphorolysis of substrate within 30 min ?
inosine + phosphate
-
Thermus thermophilus hypoxanthine + alpha-D-ribose 1-phosphate 19.1% phosphorolysis of substrate within 30 min ?
inosine + phosphate
-
Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 hypoxanthine + alpha-D-ribose 1-phosphate 19.1% phosphorolysis of substrate within 30 min ?
additional information no substrate: acyclovir, benzimidazole, 2,6-diaminopurine Thermus thermophilus ?
-
?
additional information no substrate: acyclovir, benzimidazole, 2,6-diaminopurine Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 ?
-
?

Subunits

Subunits Comment Organism
hexamer 6 * 25415, calculated Thermus thermophilus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
80
-
half-life 132 h Thermus thermophilus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
173
-
Inosine pH 7.0, 80°C Thermus thermophilus
296
-
guanosine pH 7.0, 80°C Thermus thermophilus
627
-
2'-deoxyinosine pH 7.0, 80°C Thermus thermophilus
830
-
2'-deoxyguanosine pH 7.0, 80°C Thermus thermophilus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
83.2
-
Inosine pH 7.0, 80°C Thermus thermophilus
304.3
-
2'-deoxyinosine pH 7.0, 80°C Thermus thermophilus
558.8
-
guanosine pH 7.0, 80°C Thermus thermophilus
822.5
-
2'-deoxyguanosine pH 7.0, 80°C Thermus thermophilus