Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Toxoplasma gondii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5'-methylthio-immucillin-H | - |
Plasmodium falciparum | |
deoxyimmucillin-H | - |
Plasmodium falciparum | |
deoxyimmucillin-H | - |
Toxoplasma gondii | |
immucillin-G | - |
Plasmodium falciparum | |
immucillin-G | - |
Toxoplasma gondii | |
immucillin-H | - |
Plasmodium falciparum | |
immucillin-H | - |
Toxoplasma gondii | |
additional information | insensitive to methylthio-immucillin-H | Toxoplasma gondii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0047 | - |
Inosine | pH 7.4 | Plasmodium falciparum | |
0.0094 | - |
guanosine | pH 7.4 | Plasmodium falciparum | |
0.0094 | - |
guanosine | pH 7.4 | Toxoplasma gondii | |
0.0106 | - |
5'-methylthioadenosine | pH 7.4 | Plasmodium falciparum | |
0.0131 | - |
Inosine | pH 7.4 | Toxoplasma gondii | |
0.0319 | - |
5'-methylthioadenosine | pH 7.4 | Toxoplasma gondii | |
0.091 | - |
deoxyinosine | pH 7.4 | Plasmodium falciparum | |
0.115 | - |
uridine | pH 7.4 | Plasmodium falciparum | |
0.259 | - |
deoxyinosine | pH 7.4 | Toxoplasma gondii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Plasmodium falciparum | Q8T9Z7 | - |
- |
Toxoplasma gondii | Q2HXR2 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant | Toxoplasma gondii |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
tachyzoite | - |
Toxoplasma gondii | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5'-methylthioadenosine + phosphate | - |
Plasmodium falciparum | adenine + 5-methylthio-alpha-D-ribose 1-phosphate | - |
? | |
5'-methylthioadenosine + phosphate | - |
Toxoplasma gondii | adenine + 5-methylthio-alpha-D-ribose 1-phosphate | - |
? | |
5'-methylthioinosine + phosphate | very low activity | Toxoplasma gondii | hypoxanthine + 5-methylthio-alpha-D-ribose 1-phosphate | - |
? | |
5'-methylthioinosine + phosphate | ver low activity | Plasmodium falciparum | hypoxanthine + 5-methylthio-alpha-D-ribose 1-phosphate | - |
? | |
deoxyinosine + phosphate | - |
Plasmodium falciparum | hypoxanthine + alpha-D-deoxyribose 1-phosphate | - |
? | |
deoxyinosine + phosphate | - |
Toxoplasma gondii | hypoxanthine + alpha-D-deoxyribose 1-phosphate | - |
? | |
guanosine + phosphate | - |
Plasmodium falciparum | guanine + alpha-D-ribose 1-phosphate | - |
? | |
guanosine + phosphate | - |
Toxoplasma gondii | guanine + alpha-D-ribose 1-phosphate | - |
? | |
inosine + phosphate | - |
Plasmodium falciparum | hypoxanthine + alpha-D-ribose 1-phosphate | - |
? | |
inosine + phosphate | - |
Toxoplasma gondii | hypoxanthine + alpha-D-ribose 1-phosphate | - |
? | |
uridine + phosphate | - |
Plasmodium falciparum | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PfPNP | - |
Plasmodium falciparum |
TgPNP | - |
Toxoplasma gondii |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.027 | - |
5'-methylthioadenosine | pH 7.4 | Toxoplasma gondii | |
0.09 | - |
uridine | pH 7.4 | Plasmodium falciparum | |
0.48 | - |
deoxyinosine | pH 7.4 | Toxoplasma gondii | |
0.89 | - |
deoxyinosine | pH 7.4 | Plasmodium falciparum | |
1.1 | - |
Inosine | pH 7.4 | Plasmodium falciparum | |
2.6 | - |
Inosine | pH 7.4 | Toxoplasma gondii | |
2.6 | - |
guanosine | pH 7.4 | Plasmodium falciparum | |
2.6 | - |
5'-methylthioadenosine | pH 7.4 | Plasmodium falciparum | |
3.6 | - |
guanosine | pH 7.4 | Toxoplasma gondii |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0000006 | - |
immucillin-H | pH 7.4, equilibrium inhibition constant | Plasmodium falciparum | |
0.0000009 | - |
immucillin-G | pH 7.4, equilibrium inhibition constant | Plasmodium falciparum | |
0.00000189 | - |
immucillin-G | pH 7.4, equilibrium inhibition constant | Toxoplasma gondii | |
0.00000203 | - |
immucillin-H | pH 7.4, equilibrium inhibition constant | Toxoplasma gondii | |
0.0000027 | - |
5'-methylthio-immucillin-H | pH 7.4, equilibrium inhibition constant | Plasmodium falciparum | |
0.0000074 | - |
deoxyimmucillin-H | pH 7.4, equilibrium inhibition constant | Plasmodium falciparum | |
0.00001 | - |
deoxyimmucillin-H | pH 7.4, equilibrium inhibition constant | Toxoplasma gondii | |
0.000029 | - |
immucillin-H | pH 7.4, inhibition constant during slow-onset phase | Plasmodium falciparum | |
0.00013 | - |
deoxyimmucillin-H | pH 7.4, inhibition constant during slow-onset phase | Plasmodium falciparum | |
0.000346 | - |
immucillin-G | pH 7.4, inhibition constant during slow-onset phase | Toxoplasma gondii | |
0.00045 | - |
immucillin-H | pH 7.4, inhibition constant during slow-onset phase | Toxoplasma gondii | |
0.000682 | - |
deoxyimmucillin-H | pH 7.4, inhibition constant during slow-onset phase | Toxoplasma gondii |