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Literature summary for 2.4.2.1 extracted from

  • Chaudhary, K.; Ting, L.M.; Kim, K.; Roos, D.S.
    Toxoplasma gondii purine nucleoside phosphorylase biochemical characterization, inhibitor profiles, and comparison with the Plasmodium falciparum ortholog (2006), J. Biol. Chem., 281, 25652-25658.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Toxoplasma gondii

Inhibitors

Inhibitors Comment Organism Structure
5'-methylthio-immucillin-H
-
Plasmodium falciparum
deoxyimmucillin-H
-
Plasmodium falciparum
deoxyimmucillin-H
-
Toxoplasma gondii
immucillin-G
-
Plasmodium falciparum
immucillin-G
-
Toxoplasma gondii
immucillin-H
-
Plasmodium falciparum
immucillin-H
-
Toxoplasma gondii
additional information insensitive to methylthio-immucillin-H Toxoplasma gondii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0047
-
Inosine pH 7.4 Plasmodium falciparum
0.0094
-
guanosine pH 7.4 Plasmodium falciparum
0.0094
-
guanosine pH 7.4 Toxoplasma gondii
0.0106
-
5'-methylthioadenosine pH 7.4 Plasmodium falciparum
0.0131
-
Inosine pH 7.4 Toxoplasma gondii
0.0319
-
5'-methylthioadenosine pH 7.4 Toxoplasma gondii
0.091
-
deoxyinosine pH 7.4 Plasmodium falciparum
0.115
-
uridine pH 7.4 Plasmodium falciparum
0.259
-
deoxyinosine pH 7.4 Toxoplasma gondii

Organism

Organism UniProt Comment Textmining
Plasmodium falciparum Q8T9Z7
-
-
Toxoplasma gondii Q2HXR2
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant Toxoplasma gondii

Source Tissue

Source Tissue Comment Organism Textmining
tachyzoite
-
Toxoplasma gondii
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5'-methylthioadenosine + phosphate
-
Plasmodium falciparum adenine + 5-methylthio-alpha-D-ribose 1-phosphate
-
?
5'-methylthioadenosine + phosphate
-
Toxoplasma gondii adenine + 5-methylthio-alpha-D-ribose 1-phosphate
-
?
5'-methylthioinosine + phosphate very low activity Toxoplasma gondii hypoxanthine + 5-methylthio-alpha-D-ribose 1-phosphate
-
?
5'-methylthioinosine + phosphate ver low activity Plasmodium falciparum hypoxanthine + 5-methylthio-alpha-D-ribose 1-phosphate
-
?
deoxyinosine + phosphate
-
Plasmodium falciparum hypoxanthine + alpha-D-deoxyribose 1-phosphate
-
?
deoxyinosine + phosphate
-
Toxoplasma gondii hypoxanthine + alpha-D-deoxyribose 1-phosphate
-
?
guanosine + phosphate
-
Plasmodium falciparum guanine + alpha-D-ribose 1-phosphate
-
?
guanosine + phosphate
-
Toxoplasma gondii guanine + alpha-D-ribose 1-phosphate
-
?
inosine + phosphate
-
Plasmodium falciparum hypoxanthine + alpha-D-ribose 1-phosphate
-
?
inosine + phosphate
-
Toxoplasma gondii hypoxanthine + alpha-D-ribose 1-phosphate
-
?
uridine + phosphate
-
Plasmodium falciparum ?
-
?

Synonyms

Synonyms Comment Organism
PfPNP
-
Plasmodium falciparum
TgPNP
-
Toxoplasma gondii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.027
-
5'-methylthioadenosine pH 7.4 Toxoplasma gondii
0.09
-
uridine pH 7.4 Plasmodium falciparum
0.48
-
deoxyinosine pH 7.4 Toxoplasma gondii
0.89
-
deoxyinosine pH 7.4 Plasmodium falciparum
1.1
-
Inosine pH 7.4 Plasmodium falciparum
2.6
-
Inosine pH 7.4 Toxoplasma gondii
2.6
-
guanosine pH 7.4 Plasmodium falciparum
2.6
-
5'-methylthioadenosine pH 7.4 Plasmodium falciparum
3.6
-
guanosine pH 7.4 Toxoplasma gondii

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0000006
-
immucillin-H pH 7.4, equilibrium inhibition constant Plasmodium falciparum
0.0000009
-
immucillin-G pH 7.4, equilibrium inhibition constant Plasmodium falciparum
0.00000189
-
immucillin-G pH 7.4, equilibrium inhibition constant Toxoplasma gondii
0.00000203
-
immucillin-H pH 7.4, equilibrium inhibition constant Toxoplasma gondii
0.0000027
-
5'-methylthio-immucillin-H pH 7.4, equilibrium inhibition constant Plasmodium falciparum
0.0000074
-
deoxyimmucillin-H pH 7.4, equilibrium inhibition constant Plasmodium falciparum
0.00001
-
deoxyimmucillin-H pH 7.4, equilibrium inhibition constant Toxoplasma gondii
0.000029
-
immucillin-H pH 7.4, inhibition constant during slow-onset phase Plasmodium falciparum
0.00013
-
deoxyimmucillin-H pH 7.4, inhibition constant during slow-onset phase Plasmodium falciparum
0.000346
-
immucillin-G pH 7.4, inhibition constant during slow-onset phase Toxoplasma gondii
0.00045
-
immucillin-H pH 7.4, inhibition constant during slow-onset phase Toxoplasma gondii
0.000682
-
deoxyimmucillin-H pH 7.4, inhibition constant during slow-onset phase Toxoplasma gondii