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Literature summary for 2.4.2.1 extracted from

  • Wielgus-Kutrowska, B.; Tebbe, J.; Schroder, W.; Luic, M.; Shugar, D.; Saenger, W.; Koellner, G.; Bzowska, A.
    Cellulomonas sp. purine nucleoside phosphorylase (PNP): comparison with human and E. coli enzymes (1998), Adv. Exp. Med. Biol., 431, 259-264.
    View publication on PubMed
Search for more literature for 2.4.2.1

Crystallization (Commentary)

Crystallization (Comment) Organism
-
 Cellulomonas sp.

Inhibitors

Inhibitors Comment Organism Structure
8-bromo-N(9)-acycloguanosine
-
 Cellulomonas sp.
acyclovir
-
 Cellulomonas sp.
adenosine
-
 Cellulomonas sp.
Formycin A
-
 Cellulomonas sp.
formycin B
-
 Cellulomonas sp.
guanine
-
 Cellulomonas sp.
N(7)-acycloguanosine
-
 Cellulomonas sp.
N(9)-acycloguanosine
-
 Cellulomonas sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.043
-
 Inosine
-
 Cellulomonas sp.
0.11
-
 guanosine
-
 Cellulomonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
29000
-
 4 * 29000, MALDI-MS Cellulomonas sp.
112000
-
 gel filtration Cellulomonas sp.

Organism

Organism UniProt Comment Textmining
Cellulomonas sp.
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
adenosine + phosphate no activity Cellulomonas sp. adenine + alpha-D-ribose 1-phosphate
-
 ?
guanosine + phosphate
-
 Cellulomonas sp. guanine + alpha-D-ribose 1-phosphate
-
 r
inosine + phosphate
-
 Cellulomonas sp. hypoxanthine + alpha-D-ribose 1-phosphate
-
 r

Subunits

Subunits Comment Organism
tetramer 4 * 29000, MALDI-MS Cellulomonas sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 stable up to Cellulomonas sp.
62
-
 pH 7.4, 50% inactivation Cellulomonas sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
6.5
-
 rather unstable below Cellulomonas sp.
8.5 10 stable in presence of orthophosphate Cellulomonas sp.

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0034
-
 N(9)-acycloguanosine
-
 Cellulomonas sp.
0.0036
-
 guanine
-
 Cellulomonas sp.
0.0041
-
 8-bromo-N(9)-acycloguanosine
-
 Cellulomonas sp.
0.019
-
 formycin B
-
 Cellulomonas sp.
0.062
-
 Formycin A
-
 Cellulomonas sp.
0.097
-
 N(7)-acycloguanosine
-
 Cellulomonas sp.
0.11
-
 acyclovir
-
 Cellulomonas sp.
0.16
-
 adenosine
-
 Cellulomonas sp.