Cloned (Comment) | Organism |
---|---|
expression of site-directed mutants in Escherichia coli | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
H86A | 10-25fold reduction in catalytic activity | Homo sapiens |
N243A | 100fold decrease in turnover-number compared to wild-type enzyme | Homo sapiens |
Q89A | 10-25fold reduction in catalytic activity | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
hypoxanthine | mutant enzyme Y88F | Homo sapiens | |
0.013 | - |
hypoxanthine | mutant enzyme S33A | Homo sapiens | |
0.019 | - |
hypoxanthine | mutant enzyme H86A | Homo sapiens | |
0.02 | - |
hypoxanthine | mutant enzyme R84A and H257A | Homo sapiens | |
0.022 | - |
Inosine | mutant enzymes N243A and T242A | Homo sapiens | |
0.024 | - |
hypoxanthine | mutant enzyme T242A | Homo sapiens | |
0.025 | - |
hypoxanthine | mutant enzyme F159A | Homo sapiens | |
0.03 | - |
hypoxanthine | wild-type enzyme | Homo sapiens | |
0.037 | - |
Inosine | mutant enzyme K244A | Homo sapiens | |
0.04 | - |
Inosine | wild-type enzyme | Homo sapiens | |
0.04 | - |
hypoxanthine | mutant enzyme K244A | Homo sapiens | |
0.058 | - |
Inosine | mutant enzyme Y88F | Homo sapiens | |
0.13 | - |
Inosine | mutant enzyme R84A | Homo sapiens | |
0.14 | - |
Inosine | mutant enzyme S33A | Homo sapiens | |
0.18 | - |
hypoxanthine | mutant enzyme M219A | Homo sapiens | |
0.21 | - |
Inosine | mutant enzyme H257A | Homo sapiens | |
0.22 | - |
Inosine | mutant enzyme E89A | Homo sapiens | |
0.22 | - |
phosphate | reaction with inosine, mutant enzyme T242A | Homo sapiens | |
0.28 | - |
Inosine | mutant enzyme H86A | Homo sapiens | |
0.31 | - |
Inosine | mutant enzyme F159A | Homo sapiens | |
0.57 | - |
hypoxanthine | mutant enzyme F200A | Homo sapiens | |
1 | - |
hypoxanthine | mutant enzyme E201A | Homo sapiens | |
2.1 | - |
phosphate | mutant enzyme Y88F | Homo sapiens | |
2.3 | - |
hypoxanthine | mutant enzyme N243A | Homo sapiens | |
2.5 | - |
phosphate | mutant enzymes H257A and F159A | Homo sapiens | |
2.7 | - |
phosphate | reaction with inosine, mutant enzyme K244A | Homo sapiens | |
3.5 | - |
phosphate | reaction with inosine, mutant enzyme N243A | Homo sapiens | |
3.6 | - |
phosphate | reaction with inosine, mutant enzyme S33A | Homo sapiens | |
4 | - |
phosphate | reaction with inosine, wild-type enzyme | Homo sapiens | |
4.3 | - |
phosphate | mutant enzyme M219A | Homo sapiens | |
4.5 | - |
phosphate | reaction with inosine, mutant enzyme F200A | Homo sapiens | |
4.5 | - |
phosphate | reaction with inosine, mutant enzyme R84A | Homo sapiens | |
6.1 | - |
phosphate | reaction with inosine, mutant enzyme E201A | Homo sapiens | |
8 | - |
Inosine | mutant enzyme M219A | Homo sapiens | |
8.4 | - |
Inosine | mutant enzyme E201A | Homo sapiens | |
9.2 | - |
phosphate | reaction with inosine, mutant enzyme H86A | Homo sapiens | |
19 | - |
Inosine | mutant enzyme F200A | Homo sapiens | |
91 | - |
phosphate | reaction with inosine, mutant enzyme E89A | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
wild-type and recombinant mutant enzymes | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
blood | - |
Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
inosine + phosphate | - |
Homo sapiens | hypoxanthine + alpha-D-ribose 1-phosphate | - |
r |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.06 | - |
Inosine | mutant enzyme N243A | Homo sapiens | |
0.09 | - |
phosphate | reaction with inosine, mutant enzyme N243A | Homo sapiens | |
0.5 | - |
hypoxanthine | mutant enzyme R84A | Homo sapiens | |
0.97 | - |
hypoxanthine | mutant enzyme H86A | Homo sapiens | |
1.1 | - |
Inosine | mutant enzyme S33A | Homo sapiens | |
1.1 | - |
phosphate | reaction with inosine, mutant enzyme K244A | Homo sapiens | |
1.4 | - |
hypoxanthine | mutant enzyme E201A | Homo sapiens | |
1.5 | - |
phosphate | reaction with inosine, mutant enzyme R84A | Homo sapiens | |
1.8 | - |
phosphate | reaction with inosine, mutant enzyme E201A | Homo sapiens | |
2.1 | - |
Inosine | mutant enzyme R84A | Homo sapiens | |
2.1 | - |
hypoxanthine | mutant enzyme S33A | Homo sapiens | |
4.2 | - |
Inosine | mutant enzyme E201A | Homo sapiens | |
6.2 | - |
phosphate | reaction with inosine, mutant enzyme F200A | Homo sapiens | |
9 | - |
hypoxanthine | mutant enzyme Y88F | Homo sapiens | |
9.4 | - |
Inosine | mutant enzyme F200A | Homo sapiens | |
9.4 | - |
phosphate | reaction with inosine, mutant enzyme H86A | Homo sapiens | |
10 | - |
hypoxanthine | mutant enzyme H257A | Homo sapiens | |
12 | - |
phosphate | reaction with inosine, mutant enzyme T242A | Homo sapiens | |
13 | - |
hypoxanthine | mutant enzyme N243A | Homo sapiens | |
13 | - |
phosphate | reaction with inosine, mutant enzyme H257A | Homo sapiens | |
14 | - |
hypoxanthine | mutant enzyme F200A | Homo sapiens | |
14 | - |
Inosine | mutant enzymes T242A and H86A | Homo sapiens | |
16 | - |
Inosine | mutant enzyme H257A | Homo sapiens | |
24 | - |
hypoxanthine | mutant enzyme F159A | Homo sapiens | |
30 | - |
phosphate | reaction with inosine, mutant enzyme F159A | Homo sapiens | |
32 | - |
Inosine | mutant enzyme F159A | Homo sapiens | |
41 | - |
phosphate | reaction with inosine, mutant enzyme M219A | Homo sapiens | |
46 | - |
Inosine | mutant enzyme E89A | Homo sapiens | |
49 | - |
Inosine | mutant enzyme K244A | Homo sapiens | |
52 | - |
phosphate | reaction with inosine, mutant enzyme K244A | Homo sapiens | |
52 | - |
Inosine | mutant enzyme M219A | Homo sapiens | |
53 | - |
phosphate | reaction with inosine, mutant enzyme E89A | Homo sapiens | |
54 | - |
Inosine | mutant enzyme Y88F | Homo sapiens | |
56 | - |
phosphate | wild-type enzyme | Homo sapiens | |
56 | - |
Inosine | wild-type enzyme | Homo sapiens | |
56 | - |
phosphate | reaction with inosine, mutant enzyme Y88F | Homo sapiens | |
58 | - |
hypoxanthine | mutant enzyme T242A | Homo sapiens | |
98 | - |
hypoxanthine | mutant enzyme K244A | Homo sapiens | |
110 | - |
hypoxanthine | wild-type enzyme | Homo sapiens | |
130 | - |
hypoxanthine | mutant enzyme M219A | Homo sapiens |