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Literature summary for 2.4.2.1 extracted from
- Purification and partial characterization of purine nucleoside phosphorylase from Serratia marcescens (1998), Biosci. Biotechnol. Biochem., 62, 667-671.
General Stability
| General Stability | Organism |
|---|---|
| lyophilization decreases the activity by 27% | Serratia marcescens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.19 | - |
guanosine | - |
Serratia marcescens |  |
| 0.35 | - |
deoxyguanosine | - |
Serratia marcescens | |
| 1.1 | - |
phosphate | - |
Serratia marcescens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 25817 | - |
6 * 25817, electrospray ionizing mass spectrometry | Serratia marcescens |
| 27000 | - |
6 * 27000, SDS-PAGE | Serratia marcescens |
| 170000 | - |
gel filtration | Serratia marcescens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Serratia marcescens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Serratia marcescens |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Serratia marcescens |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, pH 7.2, 10 mM Tris-HCl buffer, stable for up to 14 days | Serratia marcescens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| adenosine + phosphate | - |
Serratia marcescens | adenine + alpha-D-ribose 1-phosphate | - |
? | |
| deoxyadenosine + phosphate | - |
Serratia marcescens | alpha-D-ribose 1-phosphate + adenine | - |
? | |
| deoxyguanosine + phosphate | - |
Serratia marcescens | guanine + alpha-D-deoxyribose 1-phosphate | - |
? | |
| deoxyinosine + phosphate | - |
Serratia marcescens | hypoxanthine + alpha-D-deoxyribose 1-phosphate | - |
r | |
| guanosine + phosphate | - |
Serratia marcescens | guanine + alpha-D-ribose 1-phosphate | - |
? | |
| inosine + phosphate | - |
Serratia marcescens | hypoxanthine + alpha-D-ribose 1-phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| hexamer | 6 * 27000, SDS-PAGE | Serratia marcescens |
| hexamer | 6 * 25817, electrospray ionizing mass spectrometry | Serratia marcescens |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 42 | - |
stable up to | Serratia marcescens |
| 50 | - |
30 min, 25% loss of activity | Serratia marcescens |
| 60 | - |
30 min, 90% loss of activity, 5 mM phosphate stabilizes | Serratia marcescens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 7.7 | - |
Serratia marcescens |
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Release 2023.1 (January 2023)
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