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Literature summary for 2.4.2.1 extracted from

  • Barsacchi, D.; Cappiello, M.; Tozzi, M.G.; Del Corso, A.; Peccatori, M.; Camici, M.; Ipata, P.L.; Mura, U.
    Purine nucleoside phosphorylase from bovine lens: purification and properties (1992), Biochim. Biophys. Acta, 1160, 163-170.
    View publication on PubMed

General Stability

General Stability Organism
freezing and thawing results in complete loss of activity of the pure enzyme Bos taurus
photooxidation in presence of methylene blue is pH-dependent Bos taurus

Inhibitors

Inhibitors Comment Organism Structure
alpha-D-ribose 1-phosphate
-
Bos taurus
folate 0.05-0.1 mM, slight competitive inhibitor Bos taurus
guanine
-
Bos taurus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.014
-
guanine reaction with ribose 1-phosphate Bos taurus
0.017
-
guanine reaction with deoxyribose 1-phosphate Bos taurus
0.02
-
hypoxanthine with ribose 1-phosphate Bos taurus
0.021
-
hypoxanthine reaction with deoxyribose 1-phosphate Bos taurus
0.024
-
2'-deoxyguanosine
-
Bos taurus
0.028
-
phosphate reaction with inosine Bos taurus
0.029
-
guanosine
-
Bos taurus
0.036
-
Inosine
-
Bos taurus
0.041
-
alpha-D-ribose 1-phosphate reaction with guanine Bos taurus
0.042
-
alpha-D-ribose 1-phosphate reaction with hypoxanthine Bos taurus
0.043
-
2'-deoxyinosine
-
Bos taurus
0.129
-
alpha-D-deoxyribose 1-phosphate reaction with hypoxanthine Bos taurus
0.133
-
alpha-D-deoxyribose 1-phosphate reaction with guanine Bos taurus
0.45
-
Xanthosine
-
Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Bos taurus

Reaction

Reaction Comment Organism Reaction ID
purine ribonucleoside + phosphate = purine + alpha-D-ribose 1-phosphate rapid equilibrium random bi-bi mechanism with formation of abortive complexes Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
ocular lens
-
Bos taurus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
77.5
-
-
Bos taurus

Storage Stability

Storage Stability Organism
4°C, 10 mM sodium phosphate buffer, pH 7.0, stable for at least 4 months provided a weekly dialysis with 2 mM DTT is performed Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
deoxyguanosine + phosphate
-
Bos taurus guanine + alpha-D-deoxyribose 1-phosphate
-
?
guanosine + phosphate
-
Bos taurus guanine + alpha-D-ribose 1-phosphate
-
r
hypoxanthine + alpha-D-deoxyribose 1-phosphate
-
Bos taurus deoxyinosine + phosphate
-
r
inosine + phosphate
-
Bos taurus hypoxanthine + alpha-D-ribose 1-phosphate
-
r

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4
-
Xanthosine
-
Bos taurus
45
-
2'-deoxyguanosine
-
Bos taurus
59
-
guanosine
-
Bos taurus
85
-
2'-deoxyinosine
-
Bos taurus
100
-
alpha-D-ribose 1-phosphate reaction with hypoxanthine Bos taurus
103
-
hypoxanthine reaction with ribose 1-phosphate Bos taurus
115
-
alpha-D-deoxyribose 1-phosphate reaction with hypoxanthine Bos taurus
119
-
Inosine
-
Bos taurus
120
-
alpha-D-ribose 1-phosphate reaction with guanine Bos taurus
127
-
guanine reaction with ribose 1-phosphate Bos taurus
127
-
hypoxanthine reaction with deoxyribose 1-phosphate Bos taurus
145
-
alpha-D-deoxyribose 1-phosphate reaction with guanine Bos taurus
158
-
guanine reaction with deoxyribose 1-phosphate Bos taurus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5 8
-
Bos taurus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0065
-
guanine with guanosine as variable substrate Bos taurus
0.0085
-
alpha-D-ribose 1-phosphate with guanosine as variable substrate Bos taurus
0.025
-
guanine with phosphate as variable substrate Bos taurus
0.035
-
alpha-D-ribose 1-phosphate with phosphate as variable substrate Bos taurus