General Stability | Organism |
---|---|
freezing and thawing results in complete loss of activity of the pure enzyme | Bos taurus |
photooxidation in presence of methylene blue is pH-dependent | Bos taurus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
alpha-D-ribose 1-phosphate | - |
Bos taurus | |
folate | 0.05-0.1 mM, slight competitive inhibitor | Bos taurus | |
guanine | - |
Bos taurus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.014 | - |
guanine | reaction with ribose 1-phosphate | Bos taurus | |
0.017 | - |
guanine | reaction with deoxyribose 1-phosphate | Bos taurus | |
0.02 | - |
hypoxanthine | with ribose 1-phosphate | Bos taurus | |
0.021 | - |
hypoxanthine | reaction with deoxyribose 1-phosphate | Bos taurus | |
0.024 | - |
2'-deoxyguanosine | - |
Bos taurus | |
0.028 | - |
phosphate | reaction with inosine | Bos taurus | |
0.029 | - |
guanosine | - |
Bos taurus | |
0.036 | - |
Inosine | - |
Bos taurus | |
0.041 | - |
alpha-D-ribose 1-phosphate | reaction with guanine | Bos taurus | |
0.042 | - |
alpha-D-ribose 1-phosphate | reaction with hypoxanthine | Bos taurus | |
0.043 | - |
2'-deoxyinosine | - |
Bos taurus | |
0.129 | - |
alpha-D-deoxyribose 1-phosphate | reaction with hypoxanthine | Bos taurus | |
0.133 | - |
alpha-D-deoxyribose 1-phosphate | reaction with guanine | Bos taurus | |
0.45 | - |
Xanthosine | - |
Bos taurus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Bos taurus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
purine ribonucleoside + phosphate = purine + alpha-D-ribose 1-phosphate | rapid equilibrium random bi-bi mechanism with formation of abortive complexes | Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
ocular lens | - |
Bos taurus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
77.5 | - |
- |
Bos taurus |
Storage Stability | Organism |
---|---|
4°C, 10 mM sodium phosphate buffer, pH 7.0, stable for at least 4 months provided a weekly dialysis with 2 mM DTT is performed | Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
deoxyguanosine + phosphate | - |
Bos taurus | guanine + alpha-D-deoxyribose 1-phosphate | - |
? | |
guanosine + phosphate | - |
Bos taurus | guanine + alpha-D-ribose 1-phosphate | - |
r | |
hypoxanthine + alpha-D-deoxyribose 1-phosphate | - |
Bos taurus | deoxyinosine + phosphate | - |
r | |
inosine + phosphate | - |
Bos taurus | hypoxanthine + alpha-D-ribose 1-phosphate | - |
r |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4 | - |
Xanthosine | - |
Bos taurus | |
45 | - |
2'-deoxyguanosine | - |
Bos taurus | |
59 | - |
guanosine | - |
Bos taurus | |
85 | - |
2'-deoxyinosine | - |
Bos taurus | |
100 | - |
alpha-D-ribose 1-phosphate | reaction with hypoxanthine | Bos taurus | |
103 | - |
hypoxanthine | reaction with ribose 1-phosphate | Bos taurus | |
115 | - |
alpha-D-deoxyribose 1-phosphate | reaction with hypoxanthine | Bos taurus | |
119 | - |
Inosine | - |
Bos taurus | |
120 | - |
alpha-D-ribose 1-phosphate | reaction with guanine | Bos taurus | |
127 | - |
guanine | reaction with ribose 1-phosphate | Bos taurus | |
127 | - |
hypoxanthine | reaction with deoxyribose 1-phosphate | Bos taurus | |
145 | - |
alpha-D-deoxyribose 1-phosphate | reaction with guanine | Bos taurus | |
158 | - |
guanine | reaction with deoxyribose 1-phosphate | Bos taurus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | 8 | - |
Bos taurus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0065 | - |
guanine | with guanosine as variable substrate | Bos taurus | |
0.0085 | - |
alpha-D-ribose 1-phosphate | with guanosine as variable substrate | Bos taurus | |
0.025 | - |
guanine | with phosphate as variable substrate | Bos taurus | |
0.035 | - |
alpha-D-ribose 1-phosphate | with phosphate as variable substrate | Bos taurus |