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Literature summary for 2.4.1.99 extracted from
- Purification and characterisation of a fructosyltransferase from Rhodotorula sp. (2008), Appl. Microbiol. Biotechnol., 79, 589-596.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| CaCl2 | 69% residual activity at 10 mM | Rhodotorula sp. |  |
| Citric acid | 65% residual activity at 10 mM | Rhodotorula sp. | |
| KCl | 40% residual activity at 10 mM | Rhodotorula sp. | |
| MnCl2 | 99% residual activity at 10 mM | Rhodotorula sp. | |
| NaCl | 74% residual activity at 10 mM | Rhodotorula sp. | |
| ZnSO4 | 68% residual activity at 10 mM | Rhodotorula sp. | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Rhodotorula sp. | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| CuSO4 | 121% activity at 10 mM | Rhodotorula sp. | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 77000 | - |
2 * 77000, SDS-PAGE | Rhodotorula sp. |
| 170000 | - |
gel filtration | Rhodotorula sp. |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| sucrose + sucrose | Rhodotorula sp. | - |
D-glucose + 1-kestose | - |
? | |
| sucrose + sucrose | Rhodotorula sp. LEB-V10 | - |
D-glucose + 1-kestose | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rhodotorula sp. | - |
- |
- |
| Rhodotorula sp. LEB-V10 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Q sepharose column chromatography | Rhodotorula sp. |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.5 | - |
crude extract | Rhodotorula sp. |
| 75.4 | - |
after 150.8fold purification | Rhodotorula sp. |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| sucrose + sucrose | - |
Rhodotorula sp. | D-glucose + 1-kestose | - |
? | |
| sucrose + sucrose | - |
Rhodotorula sp. LEB-V10 | D-glucose + 1-kestose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | 2 * 77000, SDS-PAGE | Rhodotorula sp. |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| fructooligosaccharide-producing extracellular enzyme | - |
Rhodotorula sp. |
| fructosyl-transferase | - |
Rhodotorula sp. |
| FTA | - |
Rhodotorula sp. |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | 70 | - |
Rhodotorula sp. |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 66 | - |
very stable at temperatures below 66°C, the half-life of the enzyme decreases very quickly at temperatures above 66°C | Rhodotorula sp. |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
around pH 5.0 | Rhodotorula sp. |
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