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Literature summary for 2.4.1.99 extracted from
- Molecular characterization of sucrose:sucrose 1-fructosyltransferase (1-SST) from Agave tequilana Weber var. azul (2007), Plant Sci., 173, 478-486.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Pichia pastoris | Agave tequilana |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| computational model based on fructan 1-exohydrolase IIa, PDB entry 1st8 | Agave tequilana |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 75000 | - |
x * 75000, SDS-PAGE | Agave tequilana |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Agave tequilana | A7RDD3 | var. azul | - |
| Agave tequilana Weber | A7RDD3 | var. azul | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 231 | - |
pH 5.5, 30°C | Agave tequilana |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1-kestose | - |
Agave tequilana | nystose + D-fructose | plus sucrose as a secondary product | ? |  |
| 1-kestose | - |
Agave tequilana Weber | nystose + D-fructose | plus sucrose as a secondary product | ? | |
| sucrose + sucrose | - |
Agave tequilana | 1-kestose + D-glucose | no production of nystose or fructose from glucose | ? | |
| sucrose + sucrose | - |
Agave tequilana Weber | 1-kestose + D-glucose | no production of nystose or fructose from glucose | ? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 75000, SDS-PAGE | Agave tequilana |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 30 | - |
Agave tequilana |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
optimal stability at | Agave tequilana |
| 35 | - |
rapid inactivation above | Agave tequilana |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 4.3 | - |
sucrose | pH 5.5, 30°C | Agave tequilana | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
- |
Agave tequilana |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
optimal stability at | Agave tequilana |
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