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Literature summary for 2.4.1.99 extracted from

  • Koops, A.J.; Jonker, H.H.
    Purification and characterization of the enzymes of fructan biosynthesis in tubers of Helianthus tuberosus Colombia. II. Purification of sucrose:sucrose 1-fructosyltransferase and reconstitution of fructan synthesis in vitro with purified sucrose:sucrose 1-fructosyltransferase and fructan:fructan 1-fructosyltransferase (1996), Plant Physiol., 110, 1167-1175.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.4.1.99

Activating Compound

Activating Compound Comment Organism Structure
pyridoxal-HCl 20 mM, stimulation to 147% of the control Helianthus tuberosus
pyridoxine 20 mM, stimulation to 120% of the control Helianthus tuberosus

Inhibitors

Inhibitors Comment Organism Structure
Ag+ 1 mM, 15% inhibition Helianthus tuberosus
Cu2+ 1 mM CuSO4, 47% inhibition Helianthus tuberosus
iodoacetamide 1 mM, 10% inhibition Helianthus tuberosus
Mn2+ 5 mM 20% inhibition Helianthus tuberosus
NEM 1 mM, 10% inhibition Helianthus tuberosus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
67000
-
 gel filtration Helianthus tuberosus
90000
-
 non-denaturing PAGE Helianthus tuberosus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
sucrose + sucrose Helianthus tuberosus the enzyme is involved in fructan biosynthesis D-glucose + 1F-beta-D-fructosylsucrose
-
 ?

Organism

Organism UniProt Comment Textmining
Helianthus tuberosus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
tuber
-
 Helianthus tuberosus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Helianthus tuberosus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,1-nystose + 1,1-nystose self-transfructosylation at a low rate Helianthus tuberosus 1,1,1-fructosylnystose + 1-kestose
-
 ?
1-kestose + 1-kestose self-transfructosylation at a low rate Helianthus tuberosus 1,1-nystose + sucrose
-
 ?
additional information the enzyme also catalyzes the removal of the terminal fructosyl unit from both 1-kestose and 1,1-nystose, which results in the release of sucrose and 1-kestose, respectively and free fructose. The enzyme has no hydrolytic activity against sucrose Helianthus tuberosus ?
-
 ?
sucrose + sucrose
-
 Helianthus tuberosus D-glucose + 1F-beta-D-fructosylsucrose
-
 ?
sucrose + sucrose the enzyme is involved in fructan biosynthesis Helianthus tuberosus D-glucose + 1F-beta-D-fructosylsucrose
-
 ?

Subunits

Subunits Comment Organism
More electrophoresis or gel filtration under denaturing conditions yields a 27000 Da and a 55000 Da fragment Helianthus tuberosus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
20 25 synthesis of 1-kestose Helianthus tuberosus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
5 37 50% of maximal activity at 5°C and at 37°C, synthesis of 1-kestose Helianthus tuberosus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5 5
-
 Helianthus tuberosus

pH Range

pH Minimum pH Maximum Comment Organism
2.7 6.5 about 50% of maximal activity at pH 2.7 and at pH 6.5 Helianthus tuberosus