Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.4.1.91 extracted from

  • Ishikura, N.; Yang, Z.Q.; Teramoto, S.
    UDP-D-glucose:flavonol 3-O- and 7-O-glucosyl transferases from young leaves of Paederia seandens var. mairei (1993), Z. Naturforsch. C, 48, 563-569.
No PubMed abstract available
Search for more literature for 2.4.1.91

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol 14 mM, stimulates to 134% Paederia scandens

Inhibitors

Inhibitors Comment Organism Structure
Cu2+
-
 Paederia scandens
dithioerythritol
-
 Paederia scandens
iodoacetamide
-
 Paederia scandens
iodoacetate
-
 Paederia scandens
NEM
-
 Paederia scandens
PCMB
-
 Paederia scandens
Zn2+
-
 Paederia scandens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00114
-
 kaempferol
-
 Paederia scandens
0.0133
-
 UDPglucose
-
 Paederia scandens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43000
-
 gel filtration Paederia scandens

Organism

Organism UniProt Comment Textmining
Paederia scandens
-
 var. Mairei
-

Purification (Commentary)

Purification (Comment) Organism
partial Paederia scandens

Source Tissue

Source Tissue Comment Organism Textmining
leaf young Paederia scandens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Paederia scandens

Storage Stability

Storage Stability Organism
-20°C, 20 mM Tris-HCl, pH 7.5, 14 mM 2-mercaptoethanol, 10% glycerol, 15% loss of activity after 2 days Paederia scandens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-alpha-D-glucose + isorhamnetin 71% of the activity with kaempferol Paederia scandens UDP + isorhamnetin 3-O-beta-D-glucoside
-
 ?
UDPglucose + kaempferol
-
 Paederia scandens UDP + kaempferol 3-O-glucoside
-
 ?
UDPglucose + kaempferol 5,7,4'-trimethyl ether 4% of the activity with kaempferol Paederia scandens UDP + kaempferol 5,7,4'-trimethyl ether 3-O-glucoside
-
 ?
UDPglucose + myricetin 43% of the activity with kaempferol Paederia scandens UDP + myrecetin 3-O-glucoside
-
 ?
UDPglucose + quercetin 76% of the activity with kaempferol Paederia scandens UDP + quercetin 3-O-glucoside
-
 ?
UDPglucose + rhamnetin 70% of the activity with kaempferol Paederia scandens UDP + rhamnetin 3-O-glucoside
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 Tris-HCl buffer Paederia scandens
8
-
 imidazole-HCl buffer Paederia scandens