Literature summary for 2.4.1.91 extracted from

Kleinehollenhorst, G.; Behrens, H.; Pegels, G.; Strunk, N.; Wiermann, R.
Formation of flavonol 3-O-diglycosides and flavonol 3-O-triglycosides by enzyme extracts from anthers of Tulipa cv apeldoorn - characterization and activity of 3 different O-glycosyltransferases during anther development (1982), Z. Naturforsch. C, 37c, 587-599.

No PubMed abstract available

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Activating Compound

Activating Compound	Comment	Organism	Structure
2-mercaptoethanol	1 mM, activation to 120%	Tulipa hybrid cultivar
dithioerythritol	1 mM, activation to 124%	Tulipa hybrid cultivar
glutathione	1 mM, activation to 118%	Tulipa hybrid cultivar
sucrose	5%, activation to 123%	Tulipa hybrid cultivar

Inhibitors

Inhibitors	Comment	Organism	Structure
PCMB	-	Tulipa hybrid cultivar

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
40000	-	gel filtration	Tulipa hybrid cultivar

Organism

Organism	UniProt	Comment	Textmining
Tulipa hybrid cultivar	-	cv. Apeldoorn	-

Purification (Commentary)

Purification (Comment)	Organism
partial	Tulipa hybrid cultivar

Source Tissue

Source Tissue	Comment	Organism	Textmining
anther	-	Tulipa hybrid cultivar	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
UDP-alpha-D-glucose + isorhamnetin	isorhamnetin is the best acceptor	Tulipa hybrid cultivar	UDP + isorhamnetin 3-O-beta-D-glucoside	-	r
UDPgalactose + isorhamnetin	isorhamnetin is the best acceptor, 45% of the activity compared to reaction with UDPglucose	Tulipa hybrid cultivar	UDP + isorhamnetin 3-O-glucoside	-	r
UDPglucose + kaempferol	45% of the activity with isorhamnetin	Tulipa hybrid cultivar	UDP + kaempferol 3-O-glucoside	-	r
UDPglucose + kaempferol-4'-O-methylether	13% of the activity with isorhamnetin	Tulipa hybrid cultivar	UDP + ?	-	r
UDPglucose + quercetin	93% of the activity with isorhamnetin	Tulipa hybrid cultivar	UDP + quercetin 3-O-glucoside	-	r
UDPglucose + rutin	4% of the activity with isorhamnetin	Tulipa hybrid cultivar	UDP + rutin 3-O-glucoside	-	r

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8.5	9	-	Tulipa hybrid cultivar

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Release 2023.1 (January 2023)