Cloned (Comment) | Organism |
---|---|
DNA fragments coding the catalytic domain (Cd7) sequence (residues 71-322) and the C-terminal 11-amino acid deletion (Cd7DELTAC) sequence (residues 71-311) are cloned into a pET23a vector and expressed in Escherichia coli | Drosophila melanogaster |
Crystallization (Comment) | Organism |
---|---|
crystal structure of the catalytic domain of beta4Gal-T7 from Drosophila melanogaster in the presence of manganese and UDP at 1.81 A resolution is shown. In the crystal structure, a new manganese ion-binding motif is observed. Superposition of the crystal structures of beta4Gal-T7 and beta4Gal-T1 shows that the catalytic pocket and the substrate-binding sites in these proteins are similar | Drosophila melanogaster |
Protein Variants | Comment | Organism |
---|---|---|
DELTA1-70 | crystallization of a refolded Drosophila Cd7 protein coding the catalytic domain (Cd7) sequence (residues 71-322) is not successful | Drosophila melanogaster |
DELTA1-70/DELTA312-322 | a DNA fragment bearing the Cd7 catalytic domain and C-terminal 11-amino acid deletion (Cd7DELTAC) sequence (residues 71-311) shows no difference in catalytic activity or folding but crystalization is not possible | Drosophila melanogaster |
additional information | a construct bearing the N-terminal peptides from bovine Cd1 (residues 143175 (P1)) fused with peptide with the Cd7DELTAC protein (last 11 amino acids deleted) shows no impact on the catalytic activity nor the folding efficiency. This construct is used for successful crystallization | Drosophila melanogaster |
Organism | UniProt | Comment | Textmining |
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Drosophila melanogaster | - |
- |
- |
Synonyms | Comment | Organism |
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beta1,4-galactosyltransferase-7 | - |
Drosophila melanogaster |
beta4Gal-T7 | - |
Drosophila melanogaster |