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Literature summary for 2.4.1.90 extracted from

  • Ramakrishnan, B.; Qasba, P.K.
    Crystal structure of the catalytic domain of Drosophila beta1,4-Galactosyltransferase-7 (2010), J. Biol. Chem., 285, 15619-15626.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
DNA fragments coding the catalytic domain (Cd7) sequence (residues 71-322) and the C-terminal 11-amino acid deletion (Cd7DELTAC) sequence (residues 71-311) are cloned into a pET23a vector and expressed in Escherichia coli Drosophila melanogaster

Crystallization (Commentary)

Crystallization (Comment) Organism
crystal structure of the catalytic domain of beta4Gal-T7 from Drosophila melanogaster in the presence of manganese and UDP at 1.81 A resolution is shown. In the crystal structure, a new manganese ion-binding motif is observed. Superposition of the crystal structures of beta4Gal-T7 and beta4Gal-T1 shows that the catalytic pocket and the substrate-binding sites in these proteins are similar Drosophila melanogaster

Protein Variants

Protein Variants Comment Organism
DELTA1-70 crystallization of a refolded Drosophila Cd7 protein coding the catalytic domain (Cd7) sequence (residues 71-322) is not successful Drosophila melanogaster
DELTA1-70/DELTA312-322 a DNA fragment bearing the Cd7 catalytic domain and C-terminal 11-amino acid deletion (Cd7DELTAC) sequence (residues 71-311) shows no difference in catalytic activity or folding but crystalization is not possible Drosophila melanogaster
additional information a construct bearing the N-terminal peptides from bovine Cd1 (residues 143–175 (P1)) fused with peptide with the Cd7DELTAC protein (last 11 amino acids deleted) shows no impact on the catalytic activity nor the folding efficiency. This construct is used for successful crystallization Drosophila melanogaster

Organism

Organism UniProt Comment Textmining
Drosophila melanogaster
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Synonyms

Synonyms Comment Organism
beta1,4-galactosyltransferase-7
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Drosophila melanogaster
beta4Gal-T7
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Drosophila melanogaster